SREBP1 Antibody - #AF4728

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Product: SREBP1 Antibody
Catalog: AF4728
Description: Rabbit polyclonal antibody to SREBP1
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Dog, Chicken
Mol.Wt.: 122kDa; 122kD(Calculated).
Uniprot: P36956
RRID: AB_2811173

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(82%), Sheep(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
SREBP1 Antibody detects endogenous levels of total SREBP1.
RRID:
AB_2811173
Cite Format: Affinity Biosciences Cat# AF4728, RRID:AB_2811173.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;

Immunogens

Immunogen:

A synthesized peptide derived from human SREBP1, corresponding to a region within the internal amino acids.

Uniprot:

P36956(SRBP1_HUMAN) >>Visit HPA database.

Gene(ID):
SREBF1(6720)
Expression:
P36956 SRBP1_HUMAN:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Sequence:
MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
83
Horse
82
Bovine
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

PTMs:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the SREBP family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

References

1). Sirtuin 3‐mediated deacetylation of acyl‐CoA synthetase family member 3 by protocatechuic acid attenuates nonalcoholic fatty liver disease. British Journal of Pharmacology, 2020 (PubMed: 32520409) [IF=6.8]

Application: WB    Species: Mouse    Sample: livers

Figure 3. PCA promotes fatty acid metabolism through SIRT3 activation. (A-E) Western blotting and real-time PCR analysis of hepatic FASN, SREBP-1c, CPT1, Acox1 and PPARα protein (n=5) and mRNA (n=6) levels in mouse livers. *P< 0.05 vs. the WT ND group, #P< 0.05 vs. the WT HFD group, &P< 0.05 vs. the SIRT3−/− ND group. (F-I) The protein expression of FASN, SREBP-1c, CPT1, Acox1, PPARα and SIRT3 was measured by Western blotting (n=5). The mRNA levels of (J) FASN, SREBP-1c (K) CPT1, Acox1 and PPARα were measured with real-time PCR (n=6). *P< 0.05 vs. the si-Control group, #P< 0.05 vs. the si-Control+PA group, &P< 0.05 vs. the si-Control+PA+PCA group.
2). Bacillus cereus Alters Bile Acid Composition and Alleviates High-Carbohydrate Diet-Induced Hepatic Lipid Accumulation in Nile Tilapia (Oreochromis niloticus). Journal of agricultural and food chemistry, 2023 (PubMed: 36926869) [IF=5.7]
3). Alkaline Mineral Complex Water Attenuates Transportation-Induced Hepatic Lipid Metabolism Dysregulation by AMPKα-SREBP-1c/PPARα Pathways. International journal of molecular sciences, 2024 (PubMed: 39518926) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 3. Effects of AMC on the protein levels of AMPKα-SREBP-1c/PPARα-regulating hepatic lipid metabolism in transported rats. (A–D) The protein levels of phosphorylated AMP-activated protein kinase alpha (p-AMPKα)/AMPKα, AMP-activated protein kinase alpha (AMPKα), AMPKα1, AMPKα2, sterol regulatory element-binding protein-1c (SREBP-1c), and Peroxisome proliferator-activated receptor alpha (PPARα) in the liver (A) on days −3 and 0, (B) at IAT, (C) on day 3, and (D) on day 10. Rats were assigned to one of six groups: Con, TS, ABT, VBT, AAT, and VAT groups. Data are expressed as the mean ± standard error of the mean (n = 6). * mean Con group on day −3 vs. day 0; Con vs. ABT or VBT on day 0; TS vs. Con, ABT, or VBT at IAT; and TS vs. Con, ABT, VBT, AAT, or VAT on days 3 and 10; # (black) mean ABT vs. VBT on days 0, 3, and 10 and at IAT, and AAT vs. VAT on days 3 and 10; # (red) mean ABT vs. AAT and VBT vs. VAT on days 3 and 10. *, # (black), and # (red): p < 0.05; **, ## (black), and ## (red): p < 0.01.
4). E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination. International immunopharmacology, 2023 (PubMed: 37696143) [IF=4.8]

Application: WB    Species: Mouse    Sample:

Fig. 2. Siah1 overexpression exacerbates HFD-induced lipid accumulation. A. Biochemical markers of liver function in the serum of Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. B. Representative images of macroscopic examination, HE (original magnification, ×100) and oil red O (original magnification, ×400) staining of mice liver tissue obtained from Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. C. Changes in liver weights. D. Spectrometer quantification of oil red O staining. E-F. Relative protein and mRNA expression levels of Siah1 and lipid metabolic genes (SREBP1, FASN and ACC1) in mice liver tissue obtained from Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. G-H. Lipid accumulations displayed by oil red O staining (original magnification, ×200) and spectrometer quantification in Hepa1-6/Ad-Siah1 or AML12/Ad-Siah1 cells after 24 h OA/PA treatment. I-J. Relative protein and mRNA expression levels of Siah1 and lipid metabolic genes (SREBP1, FASN and ACC1) in Hepa1-6/Ad-Siah1 or (K-L) AML12/Ad-Siah1 cells after 24 h OA/PA treatment. The data are presented as mean ± SD. Student’s t-test was used to evaluate the statistical significance. *P < 0.05, **P < 0.01 vs. Ad-GFP.
5). Patchouli alcohol alleviates metabolic dysfunction-associated steatohepatitis via inhibiting mitochondria-associated endoplasmic reticulum membrane disruption-induced hepatic steatosis and inflammation in rats. International immunopharmacology, 2024 (PubMed: 38971107) [IF=4.8]
6). Si-Ni-San Reduces Hepatic Lipid Deposition in Rats with Metabolic Associated Fatty Liver Disease by AMPK/SIRT1 Pathway. Drug Design, Development and Therapy, 2023 (PubMed: 37808345) [IF=4.7]

Application: WB    Species: Rat    Sample: liver tissue

Figure 5 Effects of Si-Ni-San (SNS) on the protein expressions related to lipid metabolism. The protein expressions of FAS, CPT-1, nuclear and cytosolic SREBP-1c and PPARα were determined by Western blot. (A and B) Typical bands of Western blot, (C–F) gray value of Western blot bands. The data are stated as the means ± SD. n = 3 *vs CON group (P < 0.05), #vs MOD group (P < 0.05). Liver was obtained from control group (CON), model group (MOD), low-dose SNS group (SNS-L), high-dose SNS group (SNS-H), and metformin group (MET).
7). The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Frontiers in Physiology, 2021 (PubMed: 34588991) [IF=4.0]

Application: WB    Species: Mice    Sample: gastrocnemius

Figure 7 The expression levels of lipid metabolism-related proteins after chronic lactate and forskolin injection. (A) Western blot analysis and relative fold protein expression of lipolysis-related proteins. (B) Western blot analysis and relative fold protein expression of lipogenesis-related proteins. CP, chronic PBS treated group; CL, chronic lactate treated group; CF, chronic forskolin treated group; CD, chronic DMSO treated group; and CLF, chronic lactate and forskolin treated group. Relative expression levels were normalized to GAPDH. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. * p<0.05.
8). Taurine alleviated hepatic steatosis in oleic acid-treated-HepG2 cells and rats fed a high-fat diet. Heliyon, 2023 (PubMed: 37274675) [IF=4.0]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 1 Effect of taurine on the TG level and FA synthesis-related enzymes and factors protein level in HepG2 cells. (a) TG levels; (b, d) protein band diagram; (c, e) Relative protein level. C: control group; CT: control supplemented with taurine; M: steatotic cell model; MT: steatotic model supplemented with taurine. ∗p < 0.05 vs. C group; #p < 0.05 vs. M group.
9). circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway. Heliyon, 2023 (PubMed: 37426804) [IF=4.0]

Application: WB    Species: Mouse    Sample:

Fig. 2 Validation of the predicted genes in ox-LDL-induced mouse macrophages. A, circSnd1, miR-485-3p, and Olr1 mRNA expression verification. B, Olr1 protein expression verification. C, Verification of the expression changes in inflammatory signaling molecules possibly downstream of the circSnd1/miR-485-3p/Olr1 axis in mouse macrophages after treatment with ox-LDL. D, Changes in the expression of key proteins in lipid metabolism in ox-LDL-induced mouse macrophages. *, #p < 0.05; **, ##p < 0.01.
10). Targeting at cancer energy metabolism and lipid droplet formation as new treatment strategies for epigallocatechin-3-gallate (EGCG) in colorectal cancer cells. Journal of Functional Foods, 2021 [IF=3.8]

Application: WB    Species: Human    Sample: HCT116 cells

Fig. 3. Effects of EGCG on the expressions of the key enzymes and transcription factor of fatty acid de novo synthesis in HCT116 cells. (A) The expressions of ACLY, FASN, ACC, SCD1, and SREBP1c at the mRNA level as determined using qRT-PCR. (B-C) Western blot and densitometry analysis. The relative intensities of proteins were calculated after normalization to β-Actin. (D-E) Immunofluorescence staining and quantification of FASN, scale bar: 20 μm. Data represent the means ± SE from three independent experiments (A and C) or from three independent experiments with 2 duplicates (E). Statistical analysis was performed using one-way ANOVA with post hoc Bonferroni’s correction test. Groups containing different lowercase letters (a-e) indicate statistical significance at the level of p < 0.05.
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