MYH9 Antibody - #AF4725

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Product: MYH9 Antibody
Catalog: AF4725
Description: Rabbit polyclonal antibody to MYH9
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 226kDa; 227kD(Calculated).
Uniprot: P35579
RRID: AB_2844726

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MYH9 Antibody detects endogenous levels of total MYH9.
RRID:
AB_2844726
Cite Format: Affinity Biosciences Cat# AF4725, RRID:AB_2844726.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BDPLT 6; BDPLT6; Cellular myosin heavy chain; Cellular myosin heavy chain type A; DFNA 17; DFNA17; EPSTS; FTNS; MGC104539; MHA; MYH 2A; MYH 9; MYH2A; MYH9; MYH9_HUMAN; MYHas8; MyHC 2A; MyHC IIa; MyHC2A; MyHCIIa; MYHSA 2; MYHSA2; Myosin 9; Myosin heavy chain 9; Myosin heavy chain 9 non muscle; Myosin heavy chain; Myosin heavy chain non muscle IIa; Myosin heavy chain nonmuscle IIa; Myosin heavy polypeptide 2; Myosin heavy polypeptide 9 non muscle; Myosin-9; Myosin9; NMHC II A; NMMHC A; NMMHC II a; NMMHC II-a; NMMHC IIA; NMMHC-A; NMMHC-IIA; NMMHCA; Non muscle myosin heavy chain A; Non muscle myosin heavy chain; Non muscle myosin heavy chain II A; Non muscle myosin heavy polypeptide 9; non-muscle IIa; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; Nonmuscle myosin heavy chain A; Nonmuscle myosin heavy chain II A; type A;

Immunogens

Immunogen:
Uniprot:

P35579(MYH9_HUMAN) >>Visit HPA database.

Gene(ID):
MYH9(4627)
Expression:
P35579 MYH9_HUMAN:

In the kidney, expressed in the glomeruli. Also expressed in leukocytes.

Sequence:
MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQIILEDQNCKLAKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQVEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVGCLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSACNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKKLEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Rabbit
83
Chicken
75
Bovine
67
Sheep
67
Xenopus
50
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35579 As Substrate

Site PTM Type Enzyme
K8 Acetylation
K8 Methylation
K8 Ubiquitination
Y9 Phosphorylation
Y11 Phosphorylation
K14 Ubiquitination
K29 Acetylation
K29 Ubiquitination
K30 Acetylation
K30 Ubiquitination
S36 Phosphorylation
K38 Ubiquitination
S39 Phosphorylation
S45 Phosphorylation
K63 Acetylation
K63 Ubiquitination
K66 Ubiquitination
K69 Ubiquitination
K74 Acetylation
K79 Ubiquitination
S81 Phosphorylation
K82 Ubiquitination
T90 Phosphorylation
C91 S-Nitrosylation
K102 Acetylation
K102 Ubiquitination
Y130 Phosphorylation
S131 Phosphorylation
Y138 Phosphorylation
K139 Ubiquitination
Y151 Phosphorylation
Y158 Phosphorylation P12931 (SRC)
S160 Phosphorylation
R165 Methylation
S169 Phosphorylation
C172 S-Nitrosylation
T173 Phosphorylation
K180 Ubiquitination
K186 Ubiquitination
Y190 Phosphorylation
Y193 Phosphorylation
S196 Phosphorylation
K225 Ubiquitination
T226 Phosphorylation
K228 Ubiquitination
K237 Ubiquitination
K261 Ubiquitination
T290 Phosphorylation
K299 Acetylation
K299 Methylation
K299 Ubiquitination
S344 Phosphorylation
K355 Acetylation
K355 Ubiquitination
K356 Acetylation
K356 Ubiquitination
T360 Phosphorylation
S364 Phosphorylation
T369 Phosphorylation
K373 Ubiquitination
S375 Phosphorylation
T383 Phosphorylation
T391 Phosphorylation
Y400 Phosphorylation
K403 Acetylation
K403 Ubiquitination
K435 Acetylation
K439 Acetylation
K441 Acetylation
K536 Ubiquitination
K540 Ubiquitination
S541 Phosphorylation
K545 Acetylation
K545 Ubiquitination
T552 Phosphorylation
K555 Ubiquitination
K565 Acetylation
K565 Ubiquitination
C569 S-Nitrosylation
K580 Ubiquitination
K587 Ubiquitination
K606 Ubiquitination
K613 Acetylation
K613 Ubiquitination
S628 Phosphorylation
K637 Acetylation
K637 Ubiquitination
T638 Phosphorylation
K651 Acetylation
K651 Methylation
K651 Ubiquitination
K656 Acetylation
K656 Ubiquitination
K678 Ubiquitination
K682 Acetylation
K682 Ubiquitination
R711 Methylation
Y721 Phosphorylation
T725 Phosphorylation
S728 Phosphorylation
K731 Ubiquitination
S751 Phosphorylation
Y754 Phosphorylation
S759 Phosphorylation
K760 Acetylation
K760 Ubiquitination
T807 Phosphorylation
K810 Ubiquitination
Y819 Phosphorylation
K821 Acetylation
K821 Ubiquitination
K833 Ubiquitination
K835 Ubiquitination
S841 Phosphorylation
K850 Ubiquitination
K856 Ubiquitination
K860 Ubiquitination
T869 Phosphorylation
S876 Phosphorylation
C896 S-Nitrosylation
K910 Ubiquitination
C917 S-Nitrosylation
R923 Methylation
C931 S-Nitrosylation
K940 Ubiquitination
S957 Phosphorylation
K961 Ubiquitination
T969 Phosphorylation
K975 Ubiquitination
C988 S-Nitrosylation
K989 Ubiquitination
K992 Acetylation
R1000 Methylation
T1006 Phosphorylation
T1009 Phosphorylation
K1014 Acetylation
S1015 Phosphorylation
S1017 Phosphorylation
K1024 Acetylation
K1024 Ubiquitination
T1030 Phosphorylation
K1048 Acetylation
K1052 Ubiquitination
K1081 Acetylation
K1081 Ubiquitination
S1114 Phosphorylation
S1122 Phosphorylation
K1145 Ubiquitination
T1151 Phosphorylation
T1155 Phosphorylation
R1162 Methylation
K1181 Ubiquitination
K1193 Acetylation
K1193 Ubiquitination
S1195 Phosphorylation
K1209 Acetylation
K1209 Ubiquitination
K1212 Acetylation
K1217 Acetylation
K1219 Acetylation
K1234 Ubiquitination
K1240 Ubiquitination
S1243 Phosphorylation
K1249 Acetylation
K1249 Ubiquitination
K1260 Ubiquitination
T1269 Phosphorylation
K1274 Acetylation
K1274 Ubiquitination
K1277 Ubiquitination
T1286 Phosphorylation
S1290 Phosphorylation
S1294 Phosphorylation
K1295 Ubiquitination
K1301 Ubiquitination
S1304 Phosphorylation
S1308 Phosphorylation
K1324 Ubiquitination
S1326 Phosphorylation
S1340 Phosphorylation
K1352 Acetylation
K1352 Ubiquitination
K1357 Acetylation
K1357 Ubiquitination
K1370 Acetylation
K1370 Ubiquitination
K1371 Acetylation
S1376 Phosphorylation
T1382 Phosphorylation
K1392 Acetylation
K1392 Ubiquitination
S1398 Phosphorylation
K1404 Acetylation
Y1408 Phosphorylation
K1410 Acetylation
K1413 Ubiquitination
K1415 Ubiquitination
T1416 Phosphorylation
S1435 Phosphorylation
K1441 Acetylation
K1442 Acetylation
K1442 Methylation
K1444 Acetylation
K1445 Acetylation
K1445 Methylation
K1445 Ubiquitination
K1454 Acetylation
K1454 Ubiquitination
K1459 Acetylation
K1459 Ubiquitination
Y1460 Phosphorylation
K1477 Acetylation
K1477 Ubiquitination
S1480 Phosphorylation
K1492 Acetylation
K1492 Ubiquitination
S1511 Phosphorylation
K1513 Ubiquitination
S1519 Phosphorylation
K1525 Acetylation
S1526 Phosphorylation
T1551 Phosphorylation
K1555 Ubiquitination
K1566 Ubiquitination
S1580 Phosphorylation
K1584 Acetylation
K1585 Acetylation
S1606 Phosphorylation
K1613 Ubiquitination
K1614 Ubiquitination
S1628 Phosphorylation
K1638 Acetylation
K1642 Ubiquitination
K1648 Ubiquitination
K1669 Ubiquitination
S1677 Phosphorylation
S1713 Phosphorylation
S1714 Phosphorylation
K1716 Ubiquitination
K1724 Acetylation
T1745 Phosphorylation
K1754 Ubiquitination
K1775 Ubiquitination
K1793 Acetylation
T1800 Phosphorylation Q96QT4 (TRPM7)
S1803 Phosphorylation Q96QT4 (TRPM7)
K1804 Ubiquitination
Y1805 Phosphorylation
K1806 Ubiquitination
S1808 Phosphorylation Q96QT4 (TRPM7)
T1810 Phosphorylation
K1815 Ubiquitination
K1828 Acetylation
K1828 Ubiquitination
K1845 Acetylation
Y1861 Phosphorylation
K1862 Acetylation
K1867 Acetylation
S1892 Phosphorylation
T1904 Phosphorylation
T1906 Phosphorylation
S1915 Phosphorylation
S1916 Phosphorylation P17252 (PRKCA)
K1918 Methylation
K1918 Ubiquitination
K1920 Methylation
R1923 Methylation
S1943 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.

PTMs:

ISGylated.

Ubiquitination.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex.
Note: Colocalizes with actin filaments at lamellipodia margins and at the leading edge of migrating cells (PubMed:20052411). In retinal pigment epithelial cells, predominantly localized to stress fiber-like structures with some localization to cytoplasmic puncta (PubMed:27331610).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In the kidney, expressed in the glomeruli. Also expressed in leukocytes.

Subunit Structure:

Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1 (By similarity). Interacts with DDR1 (By similarity). Interacts with SLC6A4 (By similarity). Interacts with PDLIM2 (By similarity). Interacts with SVIL. Interacts with HTRA3. Interacts with Myo7a (By similarity). Interacts with C9orf135. Interacts with LIMCH1; independently of the integration of MYH9 into the myosin complex. Interacts with RAB3A.

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

References

1). USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma. Theranostics, 2019 (PubMed: 30809294) [IF=12.4]

Application: WB    Species: human    Sample: PLC-PRF-5 and Hep3B cells

Figure 6.| Formononetin inhibits EMT of hepatocellular carcinoma. (A) PLC-PRF-5 and Hep3B cells were treated with different concentrations of Formononetin. Cellular extracts were prepared for CHIP assays with anti-SLUG. (B) PLC-PRF-5 and Hep3B cells were transfected with E-cadherin - dependent reporter gene plasmids. Luciferase activity was measured under 20μM, 40μM Formononetin treatment. *P<0.05, **P<0.01, one-way ANOVA. (C) Cell phenotype changes of PLC-PRF-5 cell treated with 20μM Formononetin. Scale bar, 10 µm. (D) Western blot analysis USP5, SLUG and EMT related markers in PLC-PRF-5 and Hep3B cells treated with different concentrations of Formononetin.

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