CAD Antibody - #AF4715

(2)  
Product: CAD Antibody
Catalog: AF4715
Description: Rabbit polyclonal antibody to CAD
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 240kDa; 243kD(Calculated).
Uniprot: P27708
RRID: AB_2844718

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
CAD Antibody detects endogenous levels of total CAD.
RRID:
AB_2844718
Cite Format: Affinity Biosciences Cat# AF4715, RRID:AB_2844718.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD;

Immunogens

Immunogen:
Uniprot:

P27708(PYR1_HUMAN) >>Visit HPA database.

Gene(ID):
CAD(790)
Sequence:
MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27708 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S10 Phosphorylation
K127 Ubiquitination
S133 Phosphorylation
K137 Ubiquitination
K165 Ubiquitination
T166 Phosphorylation
T172 Phosphorylation
R177 Methylation
K186 Acetylation
K186 Methylation
K186 Ubiquitination
Y267 Phosphorylation
T375 Phosphorylation
K424 Ubiquitination
T456 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T475 Phosphorylation
K490 Ubiquitination
T504 Phosphorylation
T585 Phosphorylation
S586 Phosphorylation
K592 Ubiquitination
K598 Ubiquitination
Y602 Phosphorylation
K696 Ubiquitination
Y735 Phosphorylation
K739 Ubiquitination
K747 Acetylation
K747 Ubiquitination
K754 Ubiquitination
K760 Ubiquitination
S761 Phosphorylation
K778 Ubiquitination
K795 Ubiquitination
K808 Ubiquitination
K867 Ubiquitination
K874 Ubiquitination
T883 Phosphorylation
T1037 Phosphorylation P27708 (CAD)
S1038 Phosphorylation
S1044 Phosphorylation
S1061 Phosphorylation
S1069 Phosphorylation
S1073 Phosphorylation
S1112 Phosphorylation
K1119 Ubiquitination
K1127 Ubiquitination
K1177 Ubiquitination
K1206 Ubiquitination
K1211 Ubiquitination
S1220 Phosphorylation
S1222 Phosphorylation
K1228 Ubiquitination
K1262 Ubiquitination
S1267 Phosphorylation
K1301 Methylation
K1309 Ubiquitination
K1313 Ubiquitination
K1323 Ubiquitination
K1325 Ubiquitination
K1387 Ubiquitination
S1406 Phosphorylation P17612 (PRKACA)
S1407 Phosphorylation
K1411 Acetylation
K1411 Ubiquitination
S1423 Phosphorylation
K1431 Ubiquitination
K1434 Ubiquitination
K1450 Sumoylation
K1450 Ubiquitination
K1460 Ubiquitination
K1521 Ubiquitination
K1618 Ubiquitination
K1625 Ubiquitination
S1646 Phosphorylation
K1657 Ubiquitination
S1698 Phosphorylation
S1724 Phosphorylation
K1778 Ubiquitination
Y1794 Phosphorylation
Y1805 Phosphorylation
S1823 Phosphorylation
T1827 Phosphorylation
S1828 Phosphorylation
T1831 Phosphorylation
T1832 Phosphorylation
T1833 Phosphorylation
R1839 Methylation
H1856 Phosphorylation
S1859 Phosphorylation P23443 (RPS6KB1) , P17612 (PRKACA)
S1873 Phosphorylation
T1884 Phosphorylation
T1888 Phosphorylation
C1889 S-Nitrosylation
Y1890 Phosphorylation
S1900 Phosphorylation
T1906 Phosphorylation
T1914 Phosphorylation
S1920 Phosphorylation
S1938 Phosphorylation
K1953 Ubiquitination
K1961 Ubiquitination
K2036 Ubiquitination
Y2103 Phosphorylation
K2125 Ubiquitination
S2154 Phosphorylation
T2155 Phosphorylation
Y2158 Phosphorylation
K2178 Ubiquitination
Y2203 Phosphorylation
Y2212 Phosphorylation
T2220 Phosphorylation

PTMs - P27708 As Enzyme

Substrate Site Source
P27708 (CAD) T1037 Uniprot

Research Backgrounds

Function:

This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

PTMs:

Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homohexamer. Interacts with CIPC.

Family&Domains:

In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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