Parathyroid Hormone Antibody | Affinity Biosciences

IF/ICC

1/1

Product:	Parathyroid Hormone Antibody
Catalog:	AF0234
Description:	Rabbit polyclonal antibody to Parathyroid Hormone
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	16kDa; 13kD(Calculated).
Uniprot:	P01270
RRID:	AB_2833409

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(93%), Bovine(87%), Horse(93%), Rabbit(86%), Dog(80%)

Clonality:

Polyclonal

Specificity:

Parathyroid Hormone Antibody detects endogenous levels of total Parathyroid Hormone.

RRID:

AB_2833409
Cite Format: Affinity Biosciences Cat# AF0234, RRID:AB_2833409.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hPTH; Parathormone; Parathyrin; Parathyroid hormone 1; Parathyroid hormone; Prepro PTH; Preproparathyroid hormone; PTH; PTH1; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHY_HUMAN;

Immunogens

Immunogen:

Uniprot:

P01270(PTHY_HUMAN) >>Visit HPA database.

Gene(ID):

PTH(5741)

Description:

PTH PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Belongs to the parathyroid hormone family.

Sequence:

P01270 PTHY_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIPAKDMAKVMIVMLAICFLTKSDGKSVKKRSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Rabbit

Dog

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01270 As Substrate

P01270

Site	PTM Type	Source
T21	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
S34	Phosphorylation	Uniprot
S48	Phosphorylation	Uniprot

Research Backgrounds

P01270_PTHY_HUMAN

Function:

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

Subcellular Location:

Secreted.

Subunit Structure:

Interacts with PTH1R (via N-terminal extracellular domain).

Family&Domains:

Belongs to the parathyroid hormone family.

References

1). Design, semi-synthesis and bioactivity evaluation of novel podophyllotoxin derivatives as potent anti-tumor agents. Bioorganic Chemistry, 2022 (PubMed: 35661529) [IF=5.1]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Parathyroid Hormone Antibody - #AF0234