5 Lipoxygenase Antibody | Affinity Biosciences

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FIGURE 7 NDUFA4L2 regulated downstream p38-5-LO pathway may be an important mechanism for hypoxic pulmonary vascular remodelling.

Product:	5 Lipoxygenase Antibody
Catalog:	AF4699
Description:	Rabbit polyclonal antibody to 5 Lipoxygenase
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	78kDa; 78kD(Calculated).
Uniprot:	P09917
RRID:	AB_2844704

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Sheep(89%), Rabbit(89%), Dog(89%)

Clonality:

Polyclonal

Specificity:

5 Lipoxygenase Antibody detects endogenous levels of total 5 Lipoxygenase.

RRID:

AB_2844704
Cite Format: Affinity Biosciences Cat# AF4699, RRID:AB_2844704.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5 LO; 5 LOX; 5-lipoxygenase; 5-LO; 5-LOX; 5LOX; 5LPG; ALOX 5; Alox5; Arachidonate 5 lipoxygenase; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; LOX5_HUMAN; MGC163204;

Immunogens

Immunogen:

Uniprot:

P09917(LOX5_HUMAN) >>Visit HPA database.

Gene(ID):

ALOX5(240)

Sequence:

P09917 LOX5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09917 As Substrate

P09917

Site	PTM Type	Enzyme	Source
Y21	Phosphorylation		Uniprot
Y23	Phosphorylation		Uniprot
Y43	Phosphorylation		Uniprot
Y54	Phosphorylation		Uniprot
Y75	Phosphorylation		Uniprot
T87	Phosphorylation		Uniprot
Y95	Phosphorylation		Uniprot
Y101	Phosphorylation		Uniprot
T105	Phosphorylation		Uniprot
Y182	Phosphorylation		Uniprot
S183	Phosphorylation		Uniprot
T250	Phosphorylation		Uniprot
T259	Phosphorylation		Uniprot
T260	Phosphorylation		Uniprot
S266	Phosphorylation		Uniprot
S272	Phosphorylation	Q9UQM7 (CAMK2A) , P17612 (PRKACA) , P49137 (MAPKAPK2)	Uniprot
T445	Phosphorylation		Uniprot
Y446	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot
T486	Phosphorylation		Uniprot
S523	Phosphorylation		Uniprot
S524	Phosphorylation	P17612 (PRKACA)	Uniprot
K528	Acetylation		Uniprot
S532	Phosphorylation		Uniprot
S664	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S671	Phosphorylation		Uniprot

Research Backgrounds

P09917_LOX5_HUMAN

Function:

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

PTMs:

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Subcellular Location:

Cytoplasm. Nucleus matrix. Nucleus membrane>Peripheral membrane protein.
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Subunit Structure:

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity.

Family&Domains:

Belongs to the lipoxygenase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). NDUFA4L2 in smooth muscle promotes vascular remodeling in hypoxic pulmonary arterial hypertension. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33340241) [IF=5.3]

Application: WB Species: Human Sample: pulmonary artery smooth muscle cells (PASMCs)

FIGURE 7 NDUFA4L2 regulated downstream p38-5-LO pathway may be an important mechanism for hypoxic pulmonary vascular remodelling. A, After interference with NDUFA4L2, Western blotting was used to detect the expression of p-ERK/ERK, p-p38/ p38, p-ERK/ERK in hypoxic HPASMCs; (B) After interference with NDUFA4L2, Western blotting was used to detect the expression of p-5-LO/5-LO in hypoxic HPASMCs. NC, negative control; Nor, normoxia; Hyp, hypoxia. *P < .05, **P < .01. n = 6. All of the values are denoted as mean ± SEM

2). GPR146 regulates pulmonary vascular remodeling by promoting pulmonary artery smooth muscle cell proliferation through 5-lipoxygenase. European journal of pharmacology, 2023 (PubMed: 37926274) [IF=5.0]

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For Research Use Only.
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5 Lipoxygenase Antibody - #AF4699