IRAK3 Antibody - #AF4677

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Product: IRAK3 Antibody
Catalog: AF4677
Description: Rabbit polyclonal antibody to IRAK3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68kDa; 68kD(Calculated).
Uniprot: Q9Y616
RRID: AB_2844683

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 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%)
Clonality:
Polyclonal
Specificity:
IRAK3 Antibody detects endogenous levels of total IRAK3.
RRID:
AB_2844683
Cite Format: Affinity Biosciences Cat# AF4677, RRID:AB_2844683.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ASRT5; IL 1 receptor associated kinase M; IL-1 receptor-associated kinase M; IL1 receptor associated kinase M; Interleukin 1 receptor associated kinase 3; Interleukin 1 receptor associated kinase M; Interleukin-1 receptor-associated kinase 3; IRAK M; IRAK-3; IRAK-M; Irak3; IRAK3_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9Y616(IRAK3_HUMAN) >>Visit HPA database.

Gene(ID):
IRAK3(11213)
Expression:
Q9Y616 IRAK3_HUMAN:

Expressed predominantly in peripheral blood lymphocytes.

Sequence:
MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQVLQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIPEHNEKGILLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Zebrafish
88
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y616 As Substrate

Site PTM Type Enzyme
Y61 Phosphorylation
S67 Phosphorylation
S110 Phosphorylation
S115 Phosphorylation
S154 Phosphorylation
T162 Phosphorylation
T188 Phosphorylation
Y189 Phosphorylation
K199 Acetylation
K379 Ubiquitination
S398 Phosphorylation
S401 Phosphorylation
S467 Phosphorylation
S484 Phosphorylation
S510 Phosphorylation
S512 Phosphorylation

Research Backgrounds

Function:

Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.

Tissue Specificity:

Expressed predominantly in peripheral blood lymphocytes.

Family&Domains:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Research Fields

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

References

1). TREM2 modulates neuroinflammation with elevated IRAK3 expression and plays a neuroprotective role after experimental SAH in rats. Neurobiology of Disease, 2022 (PubMed: 35781003) [IF=6.1]

Application: WB    Species: Rat    Sample:

Fig. 9. TREM2 modulated neuroinflammation partly through regulating the expression of IRAK3 after SAH. A, To explore the mechanism of how TREM2 regulated inflammation, we detected the protein level of IRAK3, a negative regulatory factor of inflammation, in the basal cortex of temporal lobe by western blot at 48 h after SAH. B, Quantitative analyzes of IRAK3 expression showed IRAK3 up-regulated after SAH. TREM2 overexpression further elevated the protein level of IRAK3, while TREM2 knockdown lowered the expression of IRAK3. $P < 0.001 in SAH vs Sham, @P < 0.001 in SAH + Lv-Trem2 vs. SAH + Lv-con, &P = 0.004 in SAH + shRNA-Trem2 vs. SAH + shRNA-con; ns, P > 0.9 in SAH + Lv-con and P > 0.9 in SAH + shRNA-con vs. SAH. n = 6 per group.

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