TSC1 Antibody | Affinity Biosciences

1/3

Western blot analysis of TSC1 in lysates of HT-1080, using TSC1 Antibody(AF4609).

Western blot analysis of extracts from COS-7 cells, using TSC1 Antibody.

Western blot analysis of extracts from various samples, using TSC1 Antibody.

Product:	TSC1 Antibody
Catalog:	AF4609
Description:	Rabbit polyclonal antibody to TSC1
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Horse, Sheep, Dog, Xenopus
Mol.Wt.:	150-170kDa; 130kD(Calculated).
Uniprot:	Q92574
RRID:	AB_2844649

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Horse(100%), Sheep(100%), Dog(90%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

TSC1 Antibody detects endogenous levels of total TSC1.

RRID:

AB_2844649
Cite Format: Affinity Biosciences Cat# AF4609, RRID:AB_2844649.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Hamartin; kiaa0243; LAM; TSC; Tsc1; Tsc1 gene; TSC1_HUMAN; Tuberous sclerosis 1; Tuberous sclerosis 1 protein; tumor suppressor;

Immunogens

Immunogen:

Uniprot:

Q92574(TSC1_HUMAN) >>Visit HPA database.

Gene(ID):

TSC1(7248)

Expression:

Q92574 TSC1_HUMAN:

Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.

Sequence:

Q92574 TSC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Xenopus

100

Dog

Pig

Bovine

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92574 As Substrate

Q92574

Site	PTM Type	Enzyme	Source
Y79	Phosphorylation		Uniprot
S91	Phosphorylation		Uniprot
S102	Phosphorylation		Uniprot
S108	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
S205	Phosphorylation		Uniprot
K221	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
T267	Phosphorylation		Uniprot
S270	Phosphorylation		Uniprot
Y271	Phosphorylation		Uniprot
Y275	Phosphorylation		Uniprot
S282	Phosphorylation		Uniprot
S295	Phosphorylation		Uniprot
Y297	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
Y304	Phosphorylation		Uniprot
T310	Phosphorylation		Uniprot
Y312	Phosphorylation		Uniprot
T357	Phosphorylation		Uniprot
T360	Phosphorylation		Uniprot
T390	Phosphorylation		Uniprot
T393	Phosphorylation		Uniprot
S394	Phosphorylation		Uniprot
Y406	Phosphorylation		Uniprot
T417	Phosphorylation	P06493 (CDK1)	Uniprot
S427	Phosphorylation		Uniprot
S468	Phosphorylation		Uniprot
S472	Phosphorylation		Uniprot
K477	Ubiquitination		Uniprot
S483	Phosphorylation		Uniprot
S487	Phosphorylation	O14920 (IKBKB)	Uniprot
T490	Phosphorylation		Uniprot
S505	Phosphorylation		Uniprot
Y508	Phosphorylation		Uniprot
S511	Phosphorylation	O14920 (IKBKB)	Uniprot
S515	Phosphorylation		Uniprot
S521	Phosphorylation		Uniprot
T547	Phosphorylation		Uniprot
S579	Phosphorylation		Uniprot
S584	Phosphorylation	P06493 (CDK1)	Uniprot
S598	Phosphorylation		Uniprot
Y604	Phosphorylation		Uniprot
K662	Ubiquitination		Uniprot
K672	Ubiquitination		Uniprot
S673	Phosphorylation		Uniprot
S682	Phosphorylation		Uniprot
K725	Ubiquitination		Uniprot
K737	Ubiquitination		Uniprot
K751	Acetylation		Uniprot
K756	Ubiquitination		Uniprot
K774	Ubiquitination		Uniprot
S871	Phosphorylation		Uniprot
T873	Phosphorylation		Uniprot
T874	Phosphorylation		Uniprot
K934	Ubiquitination		Uniprot
T1020	Phosphorylation		Uniprot
S1024	Phosphorylation		Uniprot
S1025	Phosphorylation		Uniprot
S1029	Phosphorylation		Uniprot
S1030	Phosphorylation		Uniprot
R1033	Methylation		Uniprot
S1046	Phosphorylation		Uniprot
T1047	Phosphorylation	P06493 (CDK1)	Uniprot
S1080	Phosphorylation		Uniprot
S1100	Phosphorylation		Uniprot
S1102	Phosphorylation		Uniprot
S1114	Phosphorylation		Uniprot
S1116	Phosphorylation		Uniprot
S1141	Phosphorylation		Uniprot
T1144	Phosphorylation		Uniprot

Research Backgrounds

Q92574_TSC1_HUMAN

Function:

In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1.

PTMs:

Phosphorylation at Ser-505 does not affect interaction with TSC2.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.
Note: At steady state found in association with membranes.

Tissue Specificity:

Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.

Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex. Interacts (via C-terminus) with the closed form of HSP90AA1 (via the middle domain and TPR repeat-binding motif). Interacts with TSC2; the interaction stabilizes TSC2 and prevents TSC2 self-aggregation. Interacts with DOCK7. Interacts with FBXW5. Interacts with TBC1D7. Interacts with WDR45B. Interacts with RPAP3 and URI1.

Family&Domains:

The C-terminal putative coiled-coil domain is necessary for interaction with TSC2.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TSC1 Antibody - #AF4609