Occludin Antibody - #AF4605

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Product: Occludin Antibody
Catalog: AF4605
Description: Rabbit polyclonal antibody to Occludin
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse
Mol.Wt.: 23~30kD, 52~65kD; 59kD(Calculated).
Uniprot: Q16625
RRID: AB_2844646

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(80%)
Clonality:
Polyclonal
Specificity:
Occludin Antibody detects endogenous levels of total Occludin.
RRID:
AB_2844646
Cite Format: Affinity Biosciences Cat# AF4605, RRID:AB_2844646.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

Immunogens

Immunogen:
Uniprot:

Q16625(OCLN_HUMAN) >>Visit HPA database.

Gene(ID):
OCLN(100506658)
Expression:
Q16625 OCLN_HUMAN:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Sequence:
MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Sheep
70
Dog
70
Rabbit
70
Xenopus
50
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16625 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
K18 Ubiquitination
S40 Phosphorylation
Y44 Phosphorylation
S45 Phosphorylation
K276 Ubiquitination
S277 Phosphorylation
K283 Ubiquitination
Y287 Phosphorylation
K299 Ubiquitination
S302 Phosphorylation
T305 Phosphorylation
S310 Phosphorylation
S313 Phosphorylation
Y315 Phosphorylation
S321 Phosphorylation
Y325 Phosphorylation
S326 Phosphorylation
S327 Phosphorylation
K330 Ubiquitination
Y337 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
Y342 Phosphorylation
K343 Ubiquitination
S344 Phosphorylation
T345 Phosphorylation
S358 Phosphorylation
Y368 Phosphorylation
S369 Phosphorylation
S370 Phosphorylation
T376 Phosphorylation
S378 Phosphorylation
K379 Ubiquitination
T393 Phosphorylation
Y398 Phosphorylation P12931 (SRC)
T400 Phosphorylation P68400 (CSNK2A1)
Y402 Phosphorylation P12931 (SRC)
T403 Phosphorylation Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ)
T404 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B)
S408 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
T424 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ)
T438 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ)
Y443 Phosphorylation
S445 Phosphorylation
S448 Phosphorylation
S458 Phosphorylation
Y467 Phosphorylation
S471 Phosphorylation
Y474 Phosphorylation
Y481 Phosphorylation
K488 Ubiquitination
S490 Phosphorylation P05771 (PRKCB)

Research Backgrounds

Function:

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

PTMs:

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Subunit Structure:

Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.

Family&Domains:

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.

Belongs to the ELL/occludin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). LXA4 protects against hypoxic-ischemic damage in neonatal rats by reducing the inflammatory response via the IκB/NF-κB pathway. International Immunopharmacology, 2020 (PubMed: 33096360) [IF=5.6]

Application: WB    Species: rat    Sample: brain

Fig. 3. |LXA4 intervention may prevent BBB disruption following HI brain damage in neonatal rats. . (F) Protein expression level of Occludin and Claudin-5, 24 h after HI brain injury.
2). Indole-3-propionic Acid Attenuates HI-Related Blood–Brain Barrier Injury in Neonatal Rats by Modulating the PXR Signaling Pathway. ACS Chemical Neuroscience, 2022 (PubMed: 36129348) [IF=5.0]
3). Bacillus halotolerans SW207 alleviates enterotoxigenic Escherichia coli-induced inflammatory responses in weaned piglets by modulating the intestinal epithelial barrier, the TLR4/MyD88/NF-κB pathway, and intestinal microbiota. Microbiology spectrum, 2024 (PubMed: 38451226) [IF=3.7]
4). Astrocytes-Derived VEGF Exacerbates the Microvascular Damage of Late Delayed RBI. NEUROSCIENCE, 2019 (PubMed: 30910640) [IF=3.3]

Application: WB    Species: mouse    Sample: brain

Fig. 4. |Analysis of the blood–brain barrier using two-photon imaging and western blot. (E) Representative western blot showing the expression of Occludin and quanti fication of the same. Data are expressed as mean ± SEM, n = 8,*P < 0.05, **P < 0.01.

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