Phospho-VAV2 (Tyr142) Antibody | Affinity Biosciences

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Product:	Phospho-VAV2 (Tyr142) Antibody
Catalog:	AF4446
Description:	Rabbit polyclonal antibody to Phospho-VAV2 (Tyr142)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Chicken, Xenopus
Mol.Wt.:	101kDa; 101kD(Calculated).
Uniprot:	P52735
RRID:	AB_2844631

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Zebrafish(92%), Horse(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

Phospho-VAV2 (Tyr142) Antibody detects endogenous levels of VAV2 only when phosphorylated at Tyr142.

RRID:

AB_2844631
Cite Format: Affinity Biosciences Cat# AF4446, RRID:AB_2844631.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Guanine nucleotide exchange factor VAV2; Oncogene VAV2; Protein vav 2; VAV 2; Vav 2 oncogene; VAV-2; VAV2; VAV2 oncogene; VAV2_HUMAN;

Immunogens

Immunogen:

Uniprot:

P52735(VAV2_HUMAN) >>Visit HPA database.

Gene(ID):

VAV2(7410)

Expression:

P52735 VAV2_HUMAN:

Widely expressed.

Sequence:

P52735 VAV2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFLCLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMIRYMQKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSAERPERQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVGAHKECLEVIPPCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSRERSASRASSRSPASCASYNFSFLSPQGLSFASQGPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREGDVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Chicken

100

Pig

Xenopus

Zebrafish

Bovine

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52735 As Substrate

P52735

Site	PTM Type	Enzyme	Source
S91	Phosphorylation		Uniprot
K124	Ubiquitination		Uniprot
Y142	Phosphorylation	P06239 (LCK) , P00533 (EGFR)	Uniprot
Y159	Phosphorylation	P06239 (LCK) , P00533 (EGFR)	Uniprot
Y172	Phosphorylation	P06239 (LCK) , P00533 (EGFR)	Uniprot
K212	Ubiquitination		Uniprot
K385	Ubiquitination		Uniprot
K399	Ubiquitination		Uniprot
K407	Ubiquitination		Uniprot
K413	Ubiquitination		Uniprot
Y426	Phosphorylation		Uniprot
K515	Acetylation		Uniprot
K573	Ubiquitination		Uniprot
T575	Phosphorylation		Uniprot
S576	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
S626	Phosphorylation		Uniprot
S639	Phosphorylation		Uniprot
S663	Phosphorylation		Uniprot
S692	Phosphorylation		Uniprot
K718	Ubiquitination		Uniprot
K757	Ubiquitination		Uniprot
S778	Phosphorylation		Uniprot
S779	Phosphorylation		Uniprot
S781	Phosphorylation		Uniprot
S836	Phosphorylation		Uniprot

Research Backgrounds

P52735_VAV2_HUMAN

Function:

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).

PTMs:

Phosphorylated on tyrosine residues in response to FGR activation.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with SSX2IP (By similarity). Interacts with NEK3 and PRLR and this interaction is prolactin-dependent.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-VAV2 (Tyr142) Antibody - #AF4446