Phospho-Eg5 (Thr926) Antibody - #AF4445

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Product: Phospho-Eg5 (Thr926) Antibody
Catalog: AF4445
Description: Rabbit polyclonal antibody to Phospho-Eg5 (Thr926)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 120kDa; 119kD(Calculated).
Uniprot: P52732
RRID: AB_2844630

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(83%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
Phospho-Eg5 (Thr926) Antibody detects endogenous levels of Eg5 only when phosphorylated at Thr926.
RRID:
AB_2844630
Cite Format: Affinity Biosciences Cat# AF4445, RRID:AB_2844630.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EG5; HKSP; KIF11; KIF11_HUMAN; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5;

Immunogens

Immunogen:
Uniprot:

P52732(KIF11_HUMAN) >>Visit HPA database.

Gene(ID):
KIF11(3832)
Sequence:
MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVKEEYITSALESTEEKLHDAASKLLNTVEETTKDVSGLHSKLDRKKAVDQHNAEAQDIFGKNLNSLFNNMEELIKDGSSKQKAMLEVHKTLFGNLLSSSVSALDTITTVALGSLTSIPENVSTHVSQIFNMILKEQSLAAESKTVLQELINVLKTDLLSSLEMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Rabbit
92
Pig
83
Dog
83
Zebrafish
83
Chicken
83
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52732 As Substrate

Site PTM Type Enzyme
K34 Ubiquitination
S36 Phosphorylation
K60 Ubiquitination
S62 Phosphorylation
T114 Phosphorylation
S120 Phosphorylation
Y125 Phosphorylation
T126 Phosphorylation
K146 Acetylation
K197 Ubiquitination
K207 Ubiquitination
Y211 Phosphorylation
K216 Ubiquitination
T223 Phosphorylation
T226 Phosphorylation
Y231 Phosphorylation
S232 Phosphorylation
S233 Phosphorylation
S240 Phosphorylation
K257 Ubiquitination
K260 Ubiquitination
S291 Phosphorylation
T294 Phosphorylation
S323 Phosphorylation
K357 Ubiquitination
K362 Ubiquitination
K372 Acetylation
K376 Acetylation
K376 Ubiquitination
K395 Ubiquitination
Y399 Phosphorylation
S401 Phosphorylation
K444 Ubiquitination
K451 Ubiquitination
K457 Ubiquitination
T458 Phosphorylation
T463 Phosphorylation
T464 Phosphorylation
K466 Ubiquitination
K472 Ubiquitination
K477 Ubiquitination
Y480 Phosphorylation
K491 Ubiquitination
K498 Ubiquitination
T506 Phosphorylation
T507 Phosphorylation
K508 Ubiquitination
K516 Ubiquitination
K521 Ubiquitination
K536 Ubiquitination
S540 Phosphorylation
K550 Ubiquitination
K618 Ubiquitination
T643 Phosphorylation
S646 Phosphorylation
S652 Phosphorylation
K655 Ubiquitination
K659 Ubiquitination
T663 Phosphorylation
K667 Ubiquitination
K712 Ubiquitination
K715 Ubiquitination
K724 Ubiquitination
K739 Ubiquitination
K745 Ubiquitination
K757 Ubiquitination
K764 Ubiquitination
K771 Ubiquitination
R784 Methylation
K792 Ubiquitination
K803 Ubiquitination
K810 Ubiquitination
S852 Phosphorylation
S858 Phosphorylation
K875 Ubiquitination
K890 Ubiquitination
K908 Ubiquitination
K918 Ubiquitination
T923 Phosphorylation
T925 Phosphorylation
T926 Phosphorylation P06493 (CDK1)
K930 Ubiquitination
S931 Phosphorylation
Y932 Phosphorylation
Y934 Phosphorylation
S936 Phosphorylation
K952 Ubiquitination
R1025 Methylation
T1029 Phosphorylation
S1033 Phosphorylation Q9HC98 (NEK6)
K1034 Ubiquitination
K1045 Ubiquitination

Research Backgrounds

Function:

Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.

PTMs:

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).

A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

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