GKAP Antibody - #AF4779

(1)  
Product: GKAP Antibody
Catalog: AF4779
Description: Rabbit polyclonal antibody to GKAP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 90-150kDa; 42kD(Calculated).
Uniprot: Q5VSY0
RRID: AB_2844608

View similar products>>

   Size Price Inventory
 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
GKAP Antibody detects endogenous levels of total GKAP.
RRID:
AB_2844608
Cite Format: Affinity Biosciences Cat# AF4779, RRID:AB_2844608.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cGMP dependent protein kinase anchoring protein 42kDa; cGMP dependent protein kinase anchoring protein of 42 kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase anchoring protein 1; G kinase-anchoring protein 1; gkap1; GKAP1_HUMAN; GKAP42; Protein kinase anchoring protein GKAP42;

Immunogens

Immunogen:
Uniprot:

Q5VSY0(GKAP1_HUMAN) >>Visit HPA database.

Gene(ID):
GKAP1(80318)
Sequence:
MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNSESDQCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VSY0 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S8 Phosphorylation
T12 Phosphorylation
S23 Phosphorylation
S25 Phosphorylation
S27 Phosphorylation
S29 Phosphorylation
T39 Phosphorylation
S42 Phosphorylation
T44 Phosphorylation
K58 Acetylation
K79 Ubiquitination
K104 Ubiquitination
Y141 Phosphorylation
Y148 Phosphorylation
T156 Phosphorylation
S158 Phosphorylation
K219 Ubiquitination
K259 Ubiquitination
K271 Ubiquitination
K282 Ubiquitination
K295 Acetylation
K303 Acetylation
T323 Phosphorylation
T327 Phosphorylation
S328 Phosphorylation
S360 Phosphorylation

Research Backgrounds

Function:

Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced translocation of SLC2A4 to the cell membrane. Involved in TNF-induced impairment of insulin-dependent IRS1 tyrosine phosphorylation.

Subcellular Location:

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PRKG1 and IRS1.

Family&Domains:

Belongs to the GKAP1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.