Product: VAV2 Antibody
Catalog: AF4746
Description: Rabbit polyclonal antibody to VAV2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Chicken, Xenopus
Mol.Wt.: 101kDa; 101kD(Calculated).
Uniprot: P52735
RRID: AB_2844604

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(92%), Horse(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
VAV2 Antibody detects endogenous levels of total VAV2.
RRID:
AB_2844604
Cite Format: Affinity Biosciences Cat# AF4746, RRID:AB_2844604.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Guanine nucleotide exchange factor VAV2; Oncogene VAV2; Protein vav 2; VAV 2; Vav 2 oncogene; VAV-2; VAV2; VAV2 oncogene; VAV2_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P52735 VAV2_HUMAN:

Widely expressed.

Sequence:
MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFLCLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMIRYMQKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSAERPERQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVGAHKECLEVIPPCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSRERSASRASSRSPASCASYNFSFLSPQGLSFASQGPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREGDVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Chicken
100
Pig
92
Xenopus
92
Zebrafish
92
Bovine
57
Sheep
57
Dog
57
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52735 As Substrate

Site PTM Type Enzyme
S91 Phosphorylation
K124 Ubiquitination
Y142 Phosphorylation P06239 (LCK) , P00533 (EGFR)
Y159 Phosphorylation P06239 (LCK) , P00533 (EGFR)
Y172 Phosphorylation P06239 (LCK) , P00533 (EGFR)
K212 Ubiquitination
K385 Ubiquitination
K399 Ubiquitination
K407 Ubiquitination
K413 Ubiquitination
Y426 Phosphorylation
K515 Acetylation
K573 Ubiquitination
T575 Phosphorylation
S576 Phosphorylation
S583 Phosphorylation
S626 Phosphorylation
S639 Phosphorylation
S663 Phosphorylation
S692 Phosphorylation
K718 Ubiquitination
K757 Ubiquitination
S778 Phosphorylation
S779 Phosphorylation
S781 Phosphorylation
S836 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).

PTMs:

Phosphorylated on tyrosine residues in response to FGR activation.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with SSX2IP (By similarity). Interacts with NEK3 and PRLR and this interaction is prolactin-dependent.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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