IREB1 Antibody - #AF4711

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Product: IREB1 Antibody
Catalog: AF4711
Description: Rabbit polyclonal antibody to IREB1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 98kDa; 98kD(Calculated).
Uniprot: P21399
RRID: AB_2844597

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
IREB1 Antibody detects endogenous levels of total IREB1.
RRID:
AB_2844597
Cite Format: Affinity Biosciences Cat# AF4711, RRID:AB_2844597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACO 1; ACO1; ACOC_HUMAN; Aconitase 1 soluble; Aconitase; Aconitase1; Aconitate hydratase; ACONS; Citrate hydro lyase; Citrate hydro-lyase; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRE BP 1; IRE-BP 1; IREB 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; Iron responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP 1; IRP1; OTTHUMP00000045233;

Immunogens

Immunogen:
Uniprot:

P21399(ACOC_HUMAN) >>Visit HPA database.

Gene(ID):
ACO1(48)
Sequence:
MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Horse
92
Bovine
92
Sheep
92
Dog
92
Xenopus
92
Chicken
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21399 As Substrate

Site PTM Type Enzyme
K26 Ubiquitination
S30 Phosphorylation
K79 Ubiquitination
K105 Ubiquitination
K106 Ubiquitination
S138 Phosphorylation P17252 (PRKCA)
K141 Ubiquitination
K158 Ubiquitination
Y184 Phosphorylation
K250 Ubiquitination
T258 Phosphorylation
T318 Phosphorylation
K335 Ubiquitination
Y336 Phosphorylation
S383 Phosphorylation
K386 Ubiquitination
K387 Ubiquitination
K396 Ubiquitination
K400 Ubiquitination
K455 Ubiquitination
Y466 Phosphorylation
S533 Phosphorylation
K572 Ubiquitination
K580 Ubiquitination
K587 Ubiquitination
K610 Ubiquitination
K615 Ubiquitination
S622 Phosphorylation
T628 Phosphorylation
K632 Ubiquitination
S638 Phosphorylation
K639 Ubiquitination
K644 Ubiquitination
S645 Phosphorylation
S711 Phosphorylation P17252 (PRKCA)
R713 Methylation
R721 Methylation
K736 Ubiquitination
K772 Ubiquitination
K785 Ubiquitination
S799 Phosphorylation
Y800 Phosphorylation
S806 Phosphorylation
K844 Ubiquitination
K852 Ubiquitination
K857 Ubiquitination

Research Backgrounds

Function:

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210).

Family&Domains:

Belongs to the aconitase/IPM isomerase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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