eIF4A2 Antibody - #AF4673
| Product: | eIF4A2 Antibody |
| Catalog: | AF4673 |
| Description: | Rabbit polyclonal antibody to eIF4A2 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 47kDa; 46kD(Calculated). |
| Uniprot: | Q14240 |
| RRID: | AB_2844595 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4673, RRID:AB_2844595.
Fold/Unfold
ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; eIF 4A II; eIF-4A-II; EIF4A; eIF4A II; eIF4A-II; EIF4A2; EIF4F; Eukaryotic initiation factor 4A II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; Eukaryotic translation initiation factor 4A2; IF4A2_HUMAN; N-terminally processed;
Immunogens
- Q14240 IF4A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14240 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Methylation | Uniprot | |
| K55 | Sumoylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| C67 | S-Nitrosylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Methylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| R169 | Methylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| K226 | Sumoylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| R256 | Methylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K292 | Sumoylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K310 | Acetylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| R317 | Methylation | Uniprot | |
| R354 | Methylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot |
Research Backgrounds
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3 (By similarity). Interacts with EIF4E. May interact with NOM1.
(Microbial infection) Interacts with herpes simplex virus 1/HHV-1 protein Vhs.
Belongs to the DEAD box helicase family. eIF4A subfamily.
Research Fields
· Genetic Information Processing > Translation > RNA transport.
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