Product: NAKED2 Antibody
Catalog: AF4647
Description: Rabbit polyclonal antibody to NAKED2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Dog
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: Q969F2
RRID: AB_2844589

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NAKED2 Antibody detects endogenous levels of total NAKED2.
RRID:
AB_2844589
Cite Format: Affinity Biosciences Cat# AF4647, RRID:AB_2844589.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hNkd2; Naked 2; Naked-2; nkd2; NKD2_HUMAN; Protein naked cuticle homolog 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q969F2 NKD2_HUMAN:

Expressed in kidney, lung, pancreas and spleen.

Sequence:
MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Zebrafish
100
Bovine
0
Sheep
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969F2 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S18 Phosphorylation
S23 Phosphorylation
S31 Phosphorylation
K74 Ubiquitination
S176 Phosphorylation
S216 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
S230 Phosphorylation
Y247 Phosphorylation
Y256 Phosphorylation
T257 Phosphorylation
S264 Phosphorylation
S286 Phosphorylation
S299 Phosphorylation
S333 Phosphorylation
S394 Phosphorylation
Y423 Phosphorylation

Research Backgrounds

Function:

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.

PTMs:

Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.

Subcellular Location:

Cell membrane. Cytoplasm. Cytoplasmic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney, lung, pancreas and spleen.

Subunit Structure:

Interacts with DVL1, DVL2, DVL3 and PPP2R3A (By similarity). Interacts with RNF25 and TGFA (via cytoplasmic domain).

Family&Domains:

The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.

Belongs to the NKD family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

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