NAKED1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from SW480, using NAKED1 Antibody.

Product:	NAKED1 Antibody
Catalog:	AF4646
Description:	Rabbit polyclonal antibody to NAKED1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	52kDa; 52kD(Calculated).
Uniprot:	Q969G9
RRID:	AB_2844588

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NAKED1 Antibody detects endogenous levels of total NAKED1.

RRID:

AB_2844588
Cite Format: Affinity Biosciences Cat# AF4646, RRID:AB_2844588.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dvl-binding protein; Naked cuticle 1; naked cuticle 1 homolog; Naked cuticle homolog 1 (Drosophila); Naked-1; NKD; Nkd1; Protein naked cuticle homolog 1;

Immunogens

Immunogen:

Uniprot:

Q969G9(NKD1_HUMAN) >>Visit HPA database.

Gene(ID):

NKD1(85407)

Expression:

Q969G9 NKD1_HUMAN:

Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen.

Sequence:

Q969G9 NKD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGGVSGPRQLRLAGTIGRSTRELVGDVLRDTLSEEEEDDFRLEVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969G9 As Substrate

Q969G9

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S48	Phosphorylation	Uniprot
S62	Phosphorylation	Uniprot
T94	Phosphorylation	Uniprot
S99	Phosphorylation	Uniprot
S115	Phosphorylation	Uniprot
S207	Phosphorylation	Uniprot
S222	Phosphorylation	Uniprot
Y245	Phosphorylation	Uniprot
Y259	Phosphorylation	Uniprot
Y268	Phosphorylation	Uniprot
S276	Phosphorylation	Uniprot
S297	Phosphorylation	Uniprot
S317	Phosphorylation	Uniprot
T323	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
R363	Methylation	Uniprot
Y441	Phosphorylation	Uniprot

Research Backgrounds

Q969G9_NKD1_HUMAN

Function:

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.

Subcellular Location:

Cell membrane. Cytoplasm.

Tissue Specificity:

Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen.

Subunit Structure:

Interacts with DVL1, DVL2, DVL3 and PPP2R3A.

Family&Domains:

Belongs to the NKD family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

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NAKED1 Antibody - #AF4646