Product: PABP1 Antibody
Catalog: AF4639
Description: Rabbit polyclonal antibody to PABP1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Xenopus
Mol.Wt.: 71kDa; 71kD(Calculated).
Uniprot: P11940
RRID: AB_2844581

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PABP1 Antibody detects endogenous levels of total PABP1.
RRID:
AB_2844581
Cite Format: Affinity Biosciences Cat# AF4639, RRID:AB_2844581.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PAB 1; PAB1; PABP 1; PABP1; PABPC 1; PABPC1; PABPC2; PABPL1; Poly A binding protein 1; Poly A binding protein cytoplasmic 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; Polyadenylate binding protein 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P11940 PABP1_HUMAN:

Ubiquitous.

Sequence:
MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Dog
50
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11940 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S32 Phosphorylation
S51 Phosphorylation
Y54 Phosphorylation
Y56 Phosphorylation
T71 Phosphorylation
K78 Ubiquitination
K80 Ubiquitination
S92 Phosphorylation
K95 Ubiquitination
S96 Phosphorylation
K104 Acetylation
K104 Ubiquitination
K108 Acetylation
K108 Ubiquitination
K113 Ubiquitination
Y116 Phosphorylation
S120 Phosphorylation
S127 Phosphorylation
K129 Ubiquitination
K138 Ubiquitination
K157 Ubiquitination
R166 Methylation
K167 Sumoylation
K167 Ubiquitination
K188 Acetylation
K188 Methylation
K188 Ubiquitination
T191 Phosphorylation
Y194 Phosphorylation
K196 Acetylation
K196 Ubiquitination
K208 Ubiquitination
K213 Acetylation
K213 Ubiquitination
S219 Phosphorylation
K221 Ubiquitination
T224 Phosphorylation
K229 Ubiquitination
S230 Phosphorylation
K231 Ubiquitination
S237 Phosphorylation
R240 Methylation
K246 Ubiquitination
K254 Ubiquitination
K259 Acetylation
K259 Ubiquitination
Y262 Phosphorylation
K269 Ubiquitination
K279 Ubiquitination
K284 Ubiquitination
Y291 Phosphorylation
Y297 Phosphorylation
K299 Acetylation
K299 Ubiquitination
R309 Methylation
K312 Ubiquitination
S315 Phosphorylation
T319 Phosphorylation
S322 Phosphorylation
K324 Ubiquitination
K333 Ubiquitination
S341 Phosphorylation
S342 Phosphorylation
K348 Ubiquitination
T360 Phosphorylation
K361 Ubiquitination
Y364 Phosphorylation
Y382 Phosphorylation
R385 Methylation
R390 Methylation
Y400 Phosphorylation
R419 Methylation
Y422 Phosphorylation
R432 Methylation
S434 Phosphorylation
R436 Methylation
R443 Methylation
R455 Methylation
R460 Methylation
R467 Methylation
R475 Methylation
S478 Phosphorylation
R481 Methylation
R493 Methylation
T502 Phosphorylation
R506 Methylation
Y511 Phosphorylation
K512 Acetylation
K512 Methylation
K512 Ubiquitination
R518 Methylation
S591 Phosphorylation
S599 Phosphorylation
K606 Ubiquitination
K620 Ubiquitination
K625 Ubiquitination
S629 Phosphorylation
T635 Phosphorylation

Research Backgrounds

Function:

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.

(Microbial infection) Positively regulates the replication of dengue virus (DENV).

PTMs:

Phosphorylated by MAPKAPK2.

Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Stress granule.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs, which may be different from stress granules (PubMed:17289661). Shuttles between the cytoplasm and the nucleus (PubMed:9582337).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

May interact with SETX. May form homodimers. Component of a multisubunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Directly interacts with IGF2BP1. Part of a complex associated with the FOS mCRD domain and consisting of HNRPD, SYNCRIP, PAIP1 and CSDE1/UNR. Interacts with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1 and PAIP2. Interacts with PAIP1 with a 1:1 stoichiometry and with PAIP2 with a 1:2 stoichiometry. The interaction with CSDE1 is direct and RNA-independent. Found in a mRNP complex with YBX2 (By similarity). Interacts with TENT2/GLD2 (By similarity). Identified in the spliceosome C complex. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with NFX1. Interacts with PIWIL1 (By similarity). Interacts with AGO1, AGO2, GSPT1 and GSPT2 Ref.53). Interacts with LARP4B (Ref.55). Interacts (via the second and third RRM domains and the C-terminus) with PAIP2B (via central acidic portion and C-terminus). Interacts with LARP1. Interacts with SHFL. Found in a complex with SHFL and LARP1. Interacts with LARP4. Interacts with ZFC3H1 in a RNase-sensitive manner. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner. Interacts with TENT5C; the interaction has no effect on TENT5C poly(A) polymerase function. Interacts with G3BP1 and G3BP2. Interacts with DDX3X. Interacts with NXF1/TAP.

(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.

Family&Domains:

The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively.

The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1-interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively.

Belongs to the polyadenylate-binding protein type-1 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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