P2X7 Antibody | Affinity Biosciences

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FIGURE 8 Bufalin-PLGA MS suppressed the protein expression of TRPV1 and P2X7 in CCI rats.

FIGURE 9 Expressions of TRPV1 and P2X7 were visualized and quantified via immunofluorescence staining.

Product:	P2X7 Antibody
Catalog:	AF4626
Description:	Rabbit polyclonal antibody to P2X7
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	78kDa; 69kD(Calculated).
Uniprot:	Q99572
RRID:	AB_2844573

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(89%), Horse(100%), Sheep(89%), Dog(90%)

Clonality:

Polyclonal

Specificity:

P2X7 Antibody detects endogenous levels of total P2X7.

RRID:

AB_2844573
Cite Format: Affinity Biosciences Cat# AF4626, RRID:AB_2844573.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP receptor; P2rx7; P2RX7_HUMAN; P2X purinoceptor 7; P2X7; P2Z receptor; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 7;

Immunogens

Immunogen:

Uniprot:

Q99572(P2RX7_HUMAN) >>Visit HPA database.

Gene(ID):

P2RX7(5027)

Expression:

Q99572 P2RX7_HUMAN:

Widely expressed with highest levels in brain and immune tissues.

Sequence:

Q99572 P2RX7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPACCSCSDVFQYETNKVTRIQSMNYGTIKWFFHVIIFSYVCFALVSDKLYQRKEPVISSVHTKVKGIAEVKEEIVENGVKKLVHSVFDTADYTFPLQGNSFFVMTNFLKTEGQEQRLCPEYPTRRTLCSSDRGCKKGWMDPQSKGIQTGRCVVYEGNQKTCEVSAWCPIEAVEEAPRPALLNSAENFTVLIKNNIDFPGHNYTTRNILPGLNITCTFHKTQNPQCPIFRLGDIFRETGDNFSDVAIQGGIMGIEIYWDCNLDRWFHHCRPKYSFRRLDDKTTNVSLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLAAVFIDFLIDTYSSNCCRSHIYPWCKCCQPCVVNEYYYRKKCESIVEPKPTLKYVSFVDESHIRMVNQQLLGRSLQDVKGQEVPRPAMDFTDLSRLPLALHDTPPIPGQPEEIQLLRKEATPRSRDSPVWCQCGSCLPSQLPESHRCLEELCCRKKPGACITTSELFRKLVLSRHVLQFLLLYQEPLLALDVDSTNSRLRHCAYRCYATWRFGSQDMADFAILPSCCRWRIRKEFPKSEGQYSGFKSPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Dog

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99572 As Substrate

Q99572

Site	PTM Type	Source
Y13	Phosphorylation	Uniprot
N187	N-Glycosylation	Uniprot
N202	N-Glycosylation	Uniprot
N213	N-Glycosylation	Uniprot
N241	N-Glycosylation	Uniprot
N284	N-Glycosylation	Uniprot
Y343	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
T449	Phosphorylation	Uniprot
Y588	Phosphorylation	Uniprot

Research Backgrounds

Q99572_P2RX7_HUMAN

Function:

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.

PTMs:

Phosphorylation results in its inactivation.

ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.

Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed with highest levels in brain and immune tissues.

Subunit Structure:

Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB. Interacts (via C-terminus) with EMP2.

Family&Domains:

Belongs to the P2X receptor family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

References

1). Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration. Advanced Materials, 2024 (PubMed: 38295393) [IF=29.4]

2). Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model. Frontiers in Pharmacology, 2022 (PubMed: 35770074) [IF=5.6]

Application: WB Species: Rat Sample:

FIGURE 8 Bufalin-PLGA MS suppressed the protein expression of TRPV1 and P2X7 in CCI rats. (A) SDS-PAGE bands of TRPV1 and P2X7 protein in the DRG of different group rats. (B) Expression levels of TRPV1 protein in the DRG of different group rats. (C) Expression level of P2X7 protein in the DRG of different group rats. Data are expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group.

Application: IF/ICC Species: Rat Sample:

FIGURE 9 Expressions of TRPV1 and P2X7 were visualized and quantified via immunofluorescence staining. (A) Immunofluorescence detection of TRPV1 protein in the DRG; green and blue staining represent TRPV1 and DAPI, respectively. (B) Immunofluorescence detection of P2X7 protein in the DRG; green and blue staining represent P2X7 and DAPI, respectively. Data is expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group. (Scale bar = 20 µm).

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P2X7 Antibody - #AF4626