Product: Phospho-TACC3 (Ser558) Antibody
Catalog: AF4506
Description: Rabbit polyclonal antibody to Phospho-TACC3 (Ser558)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 80kDa; 90kD(Calculated).
Uniprot: Q9Y6A5
RRID: AB_2844555

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(92%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TACC3 (Ser558) Antibody detects endogenous levels of TACC3 only when phosphorylated at Ser558.
RRID:
AB_2844555
Cite Format: Affinity Biosciences Cat# AF4506, RRID:AB_2844555.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERIC 1; ERIC-1; ERIC1; MGC117382; MGC133242; OTTHUMP00000113796; TACC3; TACC3_HUMAN; Transforming acidic coiled coil containing protein 3; Transforming acidic coiled-coil-containing protein 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSLQVLNDKNVSNEKNTENCDFLFSPPEVTGRSSVLRVSQKENVPPKNLAKAMKVTFQTPLRDPQTHRILSPSMASKLEAPFTQDDTLGLENSHPVWTQKENQQLIKEVDAKTTHGILQKPVEADTDLLGDASPAFGSGSSSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGCPAGVGTPVPADGTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASSSRSGPVKLEFDVSDGATSKRAPPPRRLGERSGLKPPLRKAAVRQQKAPQEVEEDDGRSGAGEDPPMPASRGSYHLDWDKMDDPNFIPFGGDTKSGCSEAQPPESPETRLGQPAAEQLHAGPATEEPGPCLSQQLHSASAEDTPVVQLAAETPTAESKERALNSASTSLPTSCPGSEPVPTHQQGQPALELKEESFRDPAEVLGTGAEVDYLEQFGTSSFKESALRKQSLYLKFDPLLRDSPGRPVPVATETSSMHGANETPSGRPREAKLVEFDFLGALDIPVPGPPPGVPAPGGPPLSTGPIVDLLQYSQKDLDAVVKATQEENRELRSRCEELHGKNLELGKIMDRFEEVVYQAMEEVQKQKELSKAEIQKVLKEKDQLTTDLNSMEKSFSDLFKRFEKQKEVIEGYRKNEESLKKCVEDYLARITQEGQRYQALKAHAEEKLQLANEEIAQVRSKAQAEALALQASLRKEQMRIQSLEKTVEQKTKENEELTRICDDLISKMEKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
92
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6A5 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K9 Ubiquitination
S12 Phosphorylation
S25 Phosphorylation
T30 Phosphorylation
R32 Methylation
S34 Phosphorylation
S39 Phosphorylation
K51 Methylation
K54 Methylation
T56 Phosphorylation
T59 Phosphorylation
T66 Phosphorylation
S71 Phosphorylation
S73 Phosphorylation
K100 Ubiquitination
S170 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
S187 Phosphorylation
T196 Phosphorylation
C206 S-Nitrosylation
T216 Phosphorylation
T228 Phosphorylation
T249 Phosphorylation
S250 Phosphorylation
T315 Phosphorylation
S317 Phosphorylation
S402 Phosphorylation
Y403 Phosphorylation
K423 Acetylation
C426 S-Nitrosylation
S434 Phosphorylation
T437 Phosphorylation
T481 Phosphorylation
T496 Phosphorylation
S505 Phosphorylation
S524 Phosphorylation
T534 Phosphorylation
Y540 Phosphorylation
K550 Ubiquitination
S552 Phosphorylation
S558 Phosphorylation O14965 (AURKA)
Y560 Phosphorylation
K562 Ubiquitination
S570 Phosphorylation
T590 Phosphorylation
S592 Phosphorylation
K649 Ubiquitination
K668 Ubiquitination
K674 Ubiquitination
Y684 Phosphorylation
K703 Ubiquitination
K720 Ubiquitination
S723 Phosphorylation
Y739 Phosphorylation
K748 Ubiquitination
K768 Ubiquitination
K788 Ubiquitination
S799 Phosphorylation
K819 Ubiquitination
K834 Ubiquitination

Research Backgrounds

Function:

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with microtubules. Interacts with CKAP5 independently of clathrin. Interacts with CKAP5 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; TACC3 (phosphorylated at Ser-558 by AURKA) and CLTC are proposed to form a composite microtubule interaction surface. Interacts with CCDC100/CEP120. The coiled coil C-terminal region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.

Family&Domains:

Belongs to the TACC family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

References

1). A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells. Advanced Science, 2022 (PubMed: 35652200) [IF=15.1]

Application: WB    Species: Human    Sample: KG1A cells

Figure 3 Characterization of cellular response to PROTACs in AML cells. A) KG1A and Kasumi‐1 cells were stained with a carboxyfluorescein succinimidyl amino ester (CFSE) probe and cultured with AURKA PROTACs (1 × 10−6 m) for 72 h. CFSE fluorescence was analyzed by flow cytometry. MFI, mean fluorescence intensity. B) KG1A cells were treated with AURKA PROTACs (1 × 10−6 m) for 48 h. The cell cycle profile was assayed by FACS with propidium iodide (PI) staining. The cell cycle phase distribution was analyzed by FlowJo software. C) A heatmap of the relative normalized abundance of proteins in TMT‐based quantitative proteomic assays. D) TMT‐based quantitative proteomics after treatment with PROTACs (500 × 10−9 m) or the DMSO Vehicle for 6 h in KG1A cells. The top 100 decreased proteins were subjected to g:Profiler to perform gene ontology (GO) analysis. E) Gene set enrichment analysis (GSEA) of RNA‐seq data from KG1A cells treated with AURKA PROTACs (1 × 10−6 m) for 48 h. F) KG1A cells were treated with AURKA PROTACs (1 × 10−6 m) or ATRA (0.6 × 10−6 m) for 48 h. Cell surface CD34 expression was analyzed by FACS. MFI, mean fluorescence intensity. G) KG1A cells were arrested with nocodazole (0.1 µg mL−1) for 16 h followed by PROTACs or MLN8237 treatment with indicated concentration for 6 h. AURKA localization were detected by immunofluorescence. Scale bar: 5 µm. H) KG1A cells were arrested with nocodazole (0.1 µg mL−1) for 16 h followed by DMSO, PROTACs (1 × 10−6 m) or MLN8237 (50 × 10−9 m) treatment for indicated times. The expression of AURKA and related proteins were detected. Statistics, significance: one‐way ANOVA with Bonferroni correction (A,F); ns, not significant;

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