Phospho-CARD11 (Ser652) Antibody - #AF4490

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Product: Phospho-CARD11 (Ser652) Antibody
Catalog: AF4490
Description: Rabbit polyclonal antibody to Phospho-CARD11 (Ser652)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 130kDa; 133kD(Calculated).
Uniprot: Q9BXL7
RRID: AB_2844541

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 only when phosphorylated at Ser652.
RRID:
AB_2844541
Cite Format: Affinity Biosciences Cat# AF4490, RRID:AB_2844541.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

0610008L17Rik; 2410011D02Rik; Bcl10 interacting maguk protein 3; BIMP 3; BIMP3; CAR11_HUMAN; CARD 11; CARD containing MAGUK protein 3; Card maguk protein 1; CARD-containing MAGUK protein 1; CARD11; CARD11 protein; Carma 1; CARMA1; Caspase recruitment domain containing protein 11; Caspase recruitment domain family member 11; Caspase recruitment domain-containing protein 11; MGC133069;

Immunogens

Immunogen:
Uniprot:

Q9BXL7(CAR11_HUMAN) >>Visit HPA database.

Gene(ID):
CARD11(84433)
Expression:
Q9BXL7 CAR11_HUMAN:

Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.

Sequence:
MPGGGPEMDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPKKEQVLELERENEMLKTKNQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEESPEDSKYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNSFTKMQPPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHERYSFGPSSIHSSSSSHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQHTTLNGDSLTSQLTLLGGNARGSFVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVPLDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYRKLVKDMEDGLITSGDSFYIRLNLNISSQLDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLDMGTIPSYSRAQQLLLVKLQRLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLVRAFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSREKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
89
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXL7 As Substrate

Site PTM Type Enzyme
Y11 Phosphorylation
K69 Ubiquitination
S116 Phosphorylation Q9UQM7 (CAMK2A)
T117 Phosphorylation
Y202 Phosphorylation
Y208 Phosphorylation
S433 Phosphorylation
S448 Phosphorylation
S459 Phosphorylation
S466 Phosphorylation
S487 Phosphorylation
Y489 Phosphorylation
Y493 Phosphorylation
S512 Phosphorylation
S515 Phosphorylation
T532 Phosphorylation
S535 Phosphorylation
S538 Phosphorylation
S541 Phosphorylation
S547 Phosphorylation
T549 Phosphorylation
S552 Phosphorylation
S556 Phosphorylation Q92918 (MAP4K1)
S558 Phosphorylation Q92918 (MAP4K1)
S559 Phosphorylation Q04759 (PRKCQ) , P05771 (PRKCB)
S562 Phosphorylation
S572 Phosphorylation
S593 Phosphorylation
S615 Phosphorylation P48729 (CSNK1A1)
S644 Phosphorylation P05771 (PRKCB) , Q04759 (PRKCQ)
S645 Phosphorylation
S652 Phosphorylation Q04759 (PRKCQ) , P05771 (PRKCB)
K696 Ubiquitination
K703 Ubiquitination
S854 Phosphorylation
T861 Phosphorylation
T864 Phosphorylation
T871 Phosphorylation
S886 Phosphorylation
S925 Phosphorylation
S929 Phosphorylation
K939 Ubiquitination
K944 Ubiquitination
S952 Phosphorylation
Y970 Phosphorylation
S995 Phosphorylation
T1002 Phosphorylation
K1019 Ubiquitination
K1029 Ubiquitination

Research Backgrounds

Function:

Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.

PTMs:

Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.

Subcellular Location:

Cytoplasm. Membrane raft.
Note: Colocalized with DPP4 in membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.

Subunit Structure:

Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail). Interacts with BCL10; as part of a CBM (CARD11-BCL10-MALT1) complex involved in NF-kappa-B activation.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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