Phospho-BACH1/BRIP1 (Thr1133) Antibody - #AF4489

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Product: Phospho-BACH1/BRIP1 (Thr1133) Antibody
Catalog: AF4489
Description: Rabbit polyclonal antibody to Phospho-BACH1/BRIP1 (Thr1133)
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Dog, Chicken
Mol.Wt.: 145kDa; 141kD(Calculated).
Uniprot: Q9BX63
RRID: AB_2844540

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Dog(92%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
Phospho-BACH1/BRIP1 (Thr1133) Antibody detects endogenous levels of BACH1/BRIP1 only when phosphorylated at Thr1133.
RRID:
AB_2844540
Cite Format: Affinity Biosciences Cat# AF4489, RRID:AB_2844540.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase BRIP1; ATP-dependent RNA helicase BRIP1; BACH 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1; BRCA1 binding helicase like protein BACH1; BRCA1 interacting protein C terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2 associated helicase 1; BRIP 1; BRIP1; FANCJ; FANCJ_HUMAN; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ;

Immunogens

Immunogen:
Uniprot:

Q9BX63(FANCJ_HUMAN) >>Visit HPA database.

Gene(ID):
BRIP1(83990)
Expression:
Q9BX63 FANCJ_HUMAN:

Ubiquitously expressed, with highest levels in testis.

Sequence:
MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTSPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
92
Chicken
91
Zebrafish
43
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BX63 As Substrate

Site PTM Type Enzyme
Y22 Phosphorylation
K39 Ubiquitination
S77 Phosphorylation
Y110 Phosphorylation
S112 Phosphorylation
T113 Phosphorylation
S116 Phosphorylation
T124 Phosphorylation
S128 Phosphorylation
K137 Ubiquitination
S139 Phosphorylation
K142 Ubiquitination
K158 Ubiquitination
K186 Acetylation
K191 Acetylation
S197 Phosphorylation
S204 Phosphorylation
S206 Phosphorylation
K222 Ubiquitination
S226 Phosphorylation
S229 Phosphorylation
S230 Phosphorylation
T232 Phosphorylation
K254 Ubiquitination
T274 Phosphorylation
S278 Phosphorylation
K297 Ubiquitination
K345 Ubiquitination
K348 Ubiquitination
Y461 Phosphorylation
K503 Ubiquitination
S505 Phosphorylation
Y508 Phosphorylation
K510 Ubiquitination
T584 Phosphorylation
K656 Ubiquitination
Y742 Phosphorylation
K747 Ubiquitination
K764 Ubiquitination
K797 Ubiquitination
K862 Ubiquitination
S895 Phosphorylation
K897 Ubiquitination
Y916 Phosphorylation
T918 Phosphorylation
Y921 Phosphorylation
S927 Phosphorylation
S930 Phosphorylation
T953 Phosphorylation
K954 Ubiquitination
S956 Phosphorylation
K982 Ubiquitination
S988 Phosphorylation
S990 Phosphorylation
S1001 Phosphorylation
S1004 Phosphorylation
S1007 Phosphorylation
T1020 Phosphorylation
S1031 Phosphorylation
S1032 Phosphorylation
T1045 Phosphorylation
T1050 Phosphorylation
S1063 Phosphorylation
T1133 Phosphorylation
T1142 Phosphorylation
S1162 Phosphorylation
K1168 Acetylation
K1168 Ubiquitination
K1214 Ubiquitination
S1237 Phosphorylation
S1239 Phosphorylation
K1249 Acetylation

Research Backgrounds

Function:

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

PTMs:

Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.

Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels in testis.

Subunit Structure:

Binds directly to the BRCT domains of BRCA1. Interacts with the CIA complex components CIAO1, CIAO2B and MMS19.

Family&Domains:

4Fe-4S iron-sulfur-binding is required for helicase activity.

Belongs to the DEAD box helicase family. DEAH subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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