Product: Phospho-GEF H1 (Ser886) Antibody
Catalog: AF4483
Description: Rabbit polyclonal antibody to Phospho-GEF H1 (Ser886)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 120kDa; 112kD(Calculated).
Uniprot: Q92974
RRID: AB_2844534

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-GEF H1 (Ser886) Antibody detects endogenous levels of GEF H1 only when phosphorylated at Ser886.
RRID:
AB_2844534
Cite Format: Affinity Biosciences Cat# AF4483, RRID:AB_2844534.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA408978; ARHG2; ARHG2_HUMAN; ARHGEF 2; ARHGEF-2; ARHGEF2; GEF; GEF H1; GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; KIAA0651; Lbcl1; Lfc; LFP40; MGC95068; Microtubule-regulated Rho-GEF; mKIAA0651; P40; Proliferating cell nucleolar antigen p40; Protein GEF-H1; Rho guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor (GEF) 2; rho/rac guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSRIESLTRARIDRSRELASKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQKDRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92974 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S6 Phosphorylation
T8 Phosphorylation
K63 Ubiquitination
K80 Ubiquitination
K88 Ubiquitination
K95 Ubiquitination
K100 Methylation
K100 Ubiquitination
S109 Phosphorylation
T115 Phosphorylation
S121 Phosphorylation
S122 Phosphorylation
Y125 Phosphorylation
S127 Phosphorylation
S129 Phosphorylation
S133 Phosphorylation
S137 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation Q7KZI7 (MARK2)
S145 Phosphorylation
K148 Ubiquitination
S149 Phosphorylation
S151 Phosphorylation
T152 Phosphorylation
T153 Phosphorylation
S163 Phosphorylation
S172 Phosphorylation Q7KZI7 (MARK2) , O14757 (CHEK1)
S174 Phosphorylation
T175 Phosphorylation
S177 Phosphorylation
T184 Phosphorylation
S186 Phosphorylation Q7KZI7 (MARK2)
S189 Phosphorylation
K230 Ubiquitination
K235 Ubiquitination
K255 Ubiquitination
S295 Phosphorylation
S309 Phosphorylation
T310 Phosphorylation
S330 Phosphorylation
K336 Ubiquitination
K347 Ubiquitination
K353 Acetylation
K353 Ubiquitination
Y356 Phosphorylation
K376 Methylation
C383 S-Nitrosylation
T392 Phosphorylation
K393 Ubiquitination
Y394 Phosphorylation
S427 Phosphorylation
Y434 Phosphorylation
K438 Ubiquitination
T455 Phosphorylation
K460 Ubiquitination
K472 Ubiquitination
K482 Acetylation
K482 Ubiquitination
K509 Ubiquitination
Y510 Phosphorylation
K517 Ubiquitination
S519 Phosphorylation
S522 Phosphorylation
K594 Ubiquitination
K600 Ubiquitination
S643 Phosphorylation
S645 Phosphorylation
S648 Phosphorylation
K666 Ubiquitination
T679 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S691 Phosphorylation
T695 Phosphorylation
S696 Phosphorylation
S711 Phosphorylation
C715 S-Nitrosylation
S719 Phosphorylation
S721 Phosphorylation
S722 Phosphorylation
S782 Phosphorylation
K799 Acetylation
K799 Ubiquitination
T802 Phosphorylation
T830 Phosphorylation
S886 Phosphorylation Q16512 (PKN1) , O14965 (AURKA) , Q13153 (PAK1) , Q7KZI7 (MARK2) , O96013 (PAK4)
Y894 Phosphorylation
S896 Phosphorylation
S903 Phosphorylation
R904 Methylation
S932 Phosphorylation
S940 Phosphorylation
S941 Phosphorylation
T945 Phosphorylation
S947 Phosphorylation
S952 Phosphorylation
S953 Phosphorylation
S956 Phosphorylation
S960 Phosphorylation Q7KZI7 (MARK2)
S976 Phosphorylation

Research Backgrounds

Function:

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons (By similarity).

PTMs:

Phosphorylation of Ser-886 by PAK1 induces binding to protein YWHAZ, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by AURKA and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. Phosphorylated on serine, threonine and tyrosine residues in a RIPK2-dependent manner.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm. Cell junction>Tight junction. Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Ruffle membrane. Cytoplasmic vesicle.
Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560). Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex composed at least of ARHGEF2, NOD2 and RIPK2. Interacts with RIPK2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Interacts with RIPK1 and RIPK3. Interacts with YWHAZ/14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA and MAPK1. Interacts with RHOA and RAC1. Interacts with NOD1. Interacts (via the N-terminal zinc finger) with CAPN6 (via domain II). Interacts with DYNLT1 (By similarity).

Family&Domains:

The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2.

The PH domain has no affinity for phosphoinositides suggesting that it does not interact directly with membranes.

The phorbol-ester/DAG-type zinc-finger and the C-terminal coiled-coil domains (606-986) are both important for association with microtubules.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.