Phospho-PPIG (Ser376) Antibody - #AF4466

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Product: Phospho-PPIG (Ser376) Antibody
Catalog: AF4466
Description: Rabbit polyclonal antibody to Phospho-PPIG (Ser376)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110kDa; 89kD(Calculated).
Uniprot: Q13427
RRID: AB_2844523

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Phospho-PPIG (Ser376) Antibody detects endogenous levels of PPIG only when phosphorylated at Ser376.
RRID:
AB_2844523
Cite Format: Affinity Biosciences Cat# AF4466, RRID:AB_2844523.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CARS cyclophilin; CARS Cyp; CARS-cyclophilin; CARS-Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Clk-associating RS-cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G (cyclophilin G); Peptidyl prolyl isomerase G; Peptidyl-prolyl cis-trans isomerase G; Peptidyl-prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Ppig; PPIG_HUMAN; Rotamase G; RotamaseG; SCAF10; SR cyclophilin; SR cyp; SR related CTD associated factor 10; SR-cyclophilin; SR-cyp; SRcyp;

Immunogens

Immunogen:
Uniprot:

Q13427(PPIG_HUMAN) >>Visit HPA database.

Gene(ID):
PPIG(9360)
Expression:
Q13427 PPIG_HUMAN:

Ubiquitous.

Sequence:
MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRRYRTPSRSRSRDRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELNEIKENQRSPVRVKERKITDHRNVSESPNRKNEKEKKVKDHKSNSKERDIRRNSEKDDKYKNKVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRSKGRDHENVKEKEKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKDRRAQSRSRECDITKGKHSYNSRTRERSRSRDRSRRVRSRTHDRDRSRSKEYHRYREQEYRRRGRSRSRERRTPPGRSRSKDRRRRRRDSRSSEREESQSRNKDKYRNQESKSSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPFSKIKQSSQDNELKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKKFDHESSPGTDEDKSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13427 As Substrate

Site PTM Type Enzyme
K158 Ubiquitination
S173 Phosphorylation
K182 Ubiquitination
S193 Phosphorylation
S195 Phosphorylation
S196 Phosphorylation
S197 Phosphorylation
S198 Phosphorylation
S199 Phosphorylation
S200 Phosphorylation
S201 Phosphorylation
S202 Phosphorylation
S204 Phosphorylation
S206 Phosphorylation
S207 Phosphorylation
S208 Phosphorylation
S212 Phosphorylation
S214 Phosphorylation
S217 Phosphorylation
S219 Phosphorylation
T223 Phosphorylation
S254 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S259 Phosphorylation
S290 Phosphorylation
S315 Phosphorylation
Y320 Phosphorylation
T343 Phosphorylation
S345 Phosphorylation
S347 Phosphorylation
S349 Phosphorylation
S356 Phosphorylation
T358 Phosphorylation
S375 Phosphorylation
S376 Phosphorylation
S386 Phosphorylation
S397 Phosphorylation
T407 Phosphorylation
S413 Phosphorylation
S415 Phosphorylation
S431 Phosphorylation
S433 Phosphorylation
S442 Phosphorylation
K447 Acetylation
K449 Acetylation
K459 Ubiquitination
K463 Ubiquitination
S487 Phosphorylation
S489 Phosphorylation
S527 Phosphorylation
S544 Phosphorylation
S546 Phosphorylation
T552 Phosphorylation
Y558 Phosphorylation
S560 Phosphorylation
S566 Phosphorylation
S568 Phosphorylation
S572 Phosphorylation
S587 Phosphorylation
T611 Phosphorylation
S628 Phosphorylation
S630 Phosphorylation
S631 Phosphorylation
S638 Phosphorylation
S666 Phosphorylation
S673 Phosphorylation
S674 Phosphorylation
S677 Phosphorylation
S687 Phosphorylation
S690 Phosphorylation
S696 Phosphorylation
S716 Phosphorylation
S717 Phosphorylation
K720 Acetylation
K724 Acetylation
S744 Phosphorylation
S745 Phosphorylation
T748 Phosphorylation
S753 Phosphorylation

Research Backgrounds

Function:

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.

Subcellular Location:

Nucleus matrix. Nucleus speckle.
Note: Colocalizes with RNA splicing factors at nuclear speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II.

Family&Domains:

The RS domain is required for the interaction with the phosphorylated C-terminal domain of RNA polymerase II.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.