p50 Dynamitin Antibody - #AF0232

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Product: p50 Dynamitin Antibody
Catalog: AF0232
Description: Rabbit polyclonal antibody to p50 Dynamitin
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kDa; 44kD(Calculated).
Uniprot: Q13561
RRID: AB_2833407

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(82%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
p50 Dynamitin Antibody detects endogenous levels of total p50 Dynamitin.
RRID:
AB_2833407
Cite Format: Affinity Biosciences Cat# AF0232, RRID:AB_2833407.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

50 kD dynein associated polypeptide; 50 kDa dynein associated polypeptide; 50 kDa dynein-associated polypeptide; DCTN-50; DCTN2; DCTN2_HUMAN; DCTN50; dynactin 2 (p50); dynactin 2; dynactin complex 50 kD subunit; Dynactin complex 50 kDa subunit; Dynactin subunit 2; DYNAMITIN; p50 dynamitin; RBP50;

Immunogens

Immunogen:
Uniprot:

Q13561(DCTN2_HUMAN) >>Visit HPA database.

Gene(ID):
DCTN2(10540)
Description:
dynactin 2 Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135. Interacts with MAPRE1.
Sequence:
MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Dog
92
Horse
83
Bovine
83
Sheep
83
Zebrafish
82
Xenopus
70
Chicken
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13561 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K5 Ubiquitination
Y6 Phosphorylation
Y54 Phosphorylation
K65 Methylation
K65 Ubiquitination
S70 Phosphorylation
R72 Methylation
Y81 Phosphorylation
S83 Phosphorylation
Y86 Phosphorylation
T98 Phosphorylation
K102 Ubiquitination
T115 Phosphorylation
K119 Ubiquitination
K125 Ubiquitination
S127 Phosphorylation
T134 Phosphorylation
K140 Ubiquitination
K146 Ubiquitination
S152 Phosphorylation
K156 Ubiquitination
K175 Ubiquitination
T198 Phosphorylation
S202 Phosphorylation
Y207 Phosphorylation
K217 Ubiquitination
K223 Ubiquitination
T232 Phosphorylation
S249 Phosphorylation
T260 Phosphorylation
S285 Phosphorylation
K289 Ubiquitination
K297 Ubiquitination
K308 Acetylation
K308 Ubiquitination
Y313 Phosphorylation
T315 Phosphorylation
S320 Phosphorylation

Research Backgrounds

Function:

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135 (By similarity). Interacts with DYNAP. Interacts with ECPAS. Interacts with MAPRE1.

Family&Domains:

Belongs to the dynactin subunit 2 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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