Phospho-PSMC5 (Ser120) Antibody | Affinity Biosciences

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Product:	Phospho-PSMC5 (Ser120) Antibody
Catalog:	AF4452
Description:	Rabbit polyclonal antibody to Phospho-PSMC5 (Ser120)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	46kDa; 46kD(Calculated).
Uniprot:	P62195
RRID:	AB_2844511

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-PSMC5 (Ser120) Antibody detects endogenous levels of PSMC5 only when phosphorylated at S120.

RRID:

AB_2844511
Cite Format: Affinity Biosciences Cat# AF4452, RRID:AB_2844511.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

26S protease regulatory subunit 8; 26S proteasome AAA-ATPase subunit RPT6; Cim3; MSUG1 protein; p45; p45/SUG; Proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome prosome macropain 26S subunit ATPase 5; Proteasome subunit p45; PRS8_HUMAN; PSMC5; Rpt6; S8; SUG1; Tat binding protein homolog 10; TBP10; Thyroid hormone receptor interacting protein 1; Thyroid hormone receptor-interacting protein 1; Thyroid receptor interactor 1; TRIP1; TRIP1(SUG1);

Immunogens

Immunogen:

Uniprot:

P62195(PRS8_HUMAN) >>Visit HPA database.

Gene(ID):

PSMC5(5705)

Sequence:

P62195 PRS8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62195 As Substrate

P62195

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K15	Ubiquitination		Uniprot
R21	Methylation		Uniprot
Y24	Phosphorylation		Uniprot
S26	Phosphorylation		Uniprot
K27	Ubiquitination		Uniprot
K38	Ubiquitination		Uniprot
K55	Ubiquitination		Uniprot
Y72	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
K94	Acetylation		Uniprot
K94	Ubiquitination		Uniprot
K101	Ubiquitination		Uniprot
T109	Phosphorylation		Uniprot
C112	S-Nitrosylation		Uniprot
S120	Phosphorylation	P17612 (PRKACA)	Uniprot
Y121	Phosphorylation		Uniprot
K125	Ubiquitination		Uniprot
K130	Ubiquitination		Uniprot
S136	Phosphorylation		Uniprot
K142	Ubiquitination		Uniprot
T147	Phosphorylation		Uniprot
Y148	Phosphorylation		Uniprot
K156	Ubiquitination		Uniprot
K162	Ubiquitination		Uniprot
K170	Ubiquitination		Uniprot
K184	Ubiquitination		Uniprot
Y189	Phosphorylation		Uniprot
K196	Ubiquitination		Uniprot
T197	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
K222	Acetylation		Uniprot
K222	Ubiquitination		Uniprot
S244	Phosphorylation		Uniprot
S256	Phosphorylation		Uniprot
S257	Phosphorylation		Uniprot
S263	Phosphorylation		Uniprot
K287	Ubiquitination		Uniprot
K290	Ubiquitination		Uniprot
S303	Phosphorylation		Uniprot
K314	Ubiquitination		Uniprot
K330	Ubiquitination		Uniprot
K346	Ubiquitination		Uniprot
K360	Ubiquitination		Uniprot
T364	Phosphorylation		Uniprot
K389	Ubiquitination		Uniprot
K397	Ubiquitination		Uniprot
K402	Ubiquitination		Uniprot
K403	Ubiquitination		Uniprot

Research Backgrounds

P62195_PRS8_HUMAN

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Subcellular Location:

Cytoplasm. Nucleus.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC5 and few additional components. Component of a complex with USP49 and RUVBL1. Interacts with PRPF19. Interacts with TRIM5. Interacts with NDC80. Interacts with PAAF1. Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR) (By similarity). Interacts with ERCC6.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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For Research Use Only.
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Phospho-PSMC5 (Ser120) Antibody - #AF4452