Phospho-SREBP1 (Ser396) Antibody - #AF4428
| Product: | Phospho-SREBP1 (Ser396) Antibody |
| Catalog: | AF4428 |
| Description: | Rabbit polyclonal antibody to Phospho-SREBP1 (Ser396) |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 122kDa; 122kD(Calculated). |
| Uniprot: | P36956 |
| RRID: | AB_2844492 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4428, RRID:AB_2844492.
Fold/Unfold
ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;
Immunogens
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
- P36956 SRBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P36956 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T59 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S117 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) | Uniprot |
| K123 | Sumoylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| R321 | Methylation | Uniprot | |
| K324 | Acetylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K333 | Acetylation | Uniprot | |
| S337 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S338 | Phosphorylation | P57059 (SIK1) , P17612 (PRKACA) | Uniprot |
| K342 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| K398 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | P57059 (SIK1) | Uniprot |
| K418 | Sumoylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| T426 | Phosphorylation | P49841 (GSK3B) , Q16539 (MAPK14) | Uniprot |
| S430 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S434 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S439 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S448 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K470 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| Y567 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K924 | Methylation | Uniprot | |
| K924 | Ubiquitination | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| S1020 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1049 | Phosphorylation | Uniprot | |
| S1060 | Phosphorylation | Uniprot | |
| K1070 | Ubiquitination | Uniprot | |
| K1121 | Ubiquitination | Uniprot |
Research Backgrounds
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Nucleus.
Nucleus.
Nucleus.
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the SREBP family.
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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