Phospho-NUR77 (Ser351) Antibody - #AF4412

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Product: Phospho-NUR77 (Ser351) Antibody
Catalog: AF4412
Description: Rabbit polyclonal antibody to Phospho-NUR77 (Ser351)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70-80kDa; 64kD(Calculated).
Uniprot: P22736
RRID: AB_2844477

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-NUR77 (Ser351) Antibody detects endogenous levels of NUR77 only when phosphorylated at Ser351.
RRID:
AB_2844477
Cite Format: Affinity Biosciences Cat# AF4412, RRID:AB_2844477.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Early response protein NAK1; GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; Nerve growth factor IB nuclear receptor variant 1; NGFIB; NP 10; NP10; NR4A1; NR4A1_HUMAN; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear receptor subfamily 4 group A member 1; NUR77; NUR77, mouse, homolog of; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; Orphan nuclear receptor TR3; Orphan receptor tr3; Receptor NGFIB; ST 59; ST-59; ST59; Steroid receptor TR3; Testicular receptor 3; TR 3; TR3; TR3 orphan receptor;

Immunogens

Immunogen:
Uniprot:

P22736(NR4A1_HUMAN) >>Visit HPA database.

Gene(ID):
NR4A1(3164)
Expression:
P22736 NR4A1_HUMAN:

Fetal muscle and adult liver, brain and thyroid.

Sequence:
MPCIQAQYGTPAPSPGPRDHLASDPLTPEFIKPTMDLASPEAAPAAPTALPSFSTFMDGYTGEFDTFLYQLPGTVQPCSSASSSASSTSSSSATSPASASFKFEDFQVYGCYPGPLSGPVDEALSSSGSDYYGSPCSAPSPSTPSFQPPQLSPWDGSFGHFSPSQTYEGLRAWTEQLPKASGPPQPPAFFSFSPPTGPSPSLAQSPLKLFPSQATHQLGEGESYSMPTAFPGLAPTSPHLEGSGILDTPVTSTKARSGAPGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKNAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPANLLTSLVRAHLDSGPSTAKLDYSKFQELVLPHFGKEDAGDVQQFYDLLSGSLEVIRKWAEKIPGFAELSPADQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHRLQCARGFGDWIDSILAFSRSLHSLLVDVPAFACLSALVLITDRHGLQEPRRVEELQNRIASCLKEHVAAVAGEPQPASCLSRLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPPIIDKIFMDTLPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22736 As Substrate

Site PTM Type Enzyme
T10 Phosphorylation
S14 Phosphorylation
S23 Phosphorylation
T27 Phosphorylation
T48 Phosphorylation
S52 Phosphorylation
S54 Phosphorylation
T55 Phosphorylation
Y60 Phosphorylation
T61 Phosphorylation
S95 Phosphorylation P45983 (MAPK8)
K102 Sumoylation
S140 Phosphorylation
T143 Phosphorylation
S152 Phosphorylation P68400 (CSNK2A1)
S193 Phosphorylation
T196 Phosphorylation
S201 Phosphorylation
S205 Phosphorylation
S257 Phosphorylation
S263 Phosphorylation
K300 Sumoylation
K300 Ubiquitination
K307 Ubiquitination
T339 Phosphorylation
S341 Phosphorylation
S351 Phosphorylation Q15418 (RPS6KA1) , P51812 (RPS6KA3) , O75582 (RPS6KA5) , P31749 (AKT1)
S360 Phosphorylation
K381 Sumoylation
K381 Ubiquitination
K386 Ubiquitination
S431 Phosphorylation P28482 (MAPK1)
K558 Ubiquitination

Research Backgrounds

Function:

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).

PTMs:

Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.

Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.

Subcellular Location:

Nucleus. Cytoplasm. Mitochondrion.
Note: Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Fetal muscle and adult liver, brain and thyroid.

Subunit Structure:

Binds DNA as a monomer (By similarity). Interacts with GADD45GIP1. Interacts with STK11. Interacts with IFI27. Heterodimer (via DNA-binding domain) with RXRA (via C-terminus); DNA-binding of the heterodimer is enhanced by 9-cis retinoic acid. Competes for the RXRA interaction with EP300 and thereby attenuates EP300 mediated acetylation of RXRA.

Family&Domains:

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

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