Phospho-Rad17 (Ser635)[Ser646] Antibody

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Product:	Phospho-Rad17 (Ser635)[Ser646] Antibody
Catalog:	AF4388
Description:	Rabbit polyclonal antibody to Phospho-Rad17 (Ser635)[Ser646]
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	77kDa; 77kD(Calculated).
Uniprot:	O75943
RRID:	AB_2844453

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(83%), Horse(83%), Sheep(83%)

Clonality:

Polyclonal

Specificity:

Phospho-Rad17 (Ser635) Antibody detects endogenous levels of Rad17 only when phosphorylated at Ser646, which site historically referenced as Ser635.

RRID:

AB_2844453
Cite Format: Affinity Biosciences Cat# AF4388, RRID:AB_2844453.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; FLJ41520; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17; RAD17 homolog (S. pombe); RAD17 homolog; Rad17 like protein; RAD17, S. pombe, homolog of; RAD17_HUMAN; RAD17Sp; Rad24; Rad24, mouse, homolog of; Rad24, S. cerevisiae, homolog of; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog;

Immunogens

Immunogen:

Uniprot:

O75943(RAD17_HUMAN) >>Visit HPA database.

Gene(ID):

RAD17(5884)

Expression:

O75943 RAD17_HUMAN:

Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).

Sequence:

O75943 RAD17_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKTFLRPKVSSTKVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPSTLESSRFPARKRGNLSSLEQIYGLENSKEYLSENEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRTDKKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYERDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIPMRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75943 As Substrate

O75943

Site	PTM Type	Enzyme	Source
T55	Phosphorylation		Uniprot
R66	Methylation		Uniprot
S71	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
K82	Ubiquitination		Uniprot
S86	Phosphorylation		Uniprot
K107	Ubiquitination		Uniprot
K118	Ubiquitination		Uniprot
K152	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K208	Ubiquitination		Uniprot
K221	Ubiquitination		Uniprot
T304	Phosphorylation		Uniprot
K319	Ubiquitination		Uniprot
S335	Phosphorylation		Uniprot
K346	Ubiquitination		Uniprot
S359	Phosphorylation		Uniprot
S362	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
K597	Ubiquitination		Uniprot
T633	Phosphorylation		Uniprot
S646	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
S656	Phosphorylation	Q13315 (ATM) , Q13535 (ATR)	Uniprot
S678	Phosphorylation		Uniprot

PTMs - O75943 As Enzyme

O75943

Substrate	Site	Source
Q13535-1 (ATR)	S480	Uniprot
Q13535-1 (ATR)	S549	Uniprot

Research Backgrounds

O75943_RAD17_HUMAN

Function:

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

PTMs:

Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.

Subcellular Location:

Nucleus.
Note: Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

Tissue Specificity:

Subunit Structure:

Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Interacts with RAD1 and RAD9 within the RAD1-RAD9-HUS1 complex. Interacts with RAD9B, POLE, SNU13 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the RAD1-RAD9-HUS1 complex.

Family&Domains:

Belongs to the rad17/RAD24 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-Rad17 (Ser635)[Ser646] Antibody - #AF4388