Phospho-DOK2 (Tyr351)[Tyr345] Antibody - #AF4383

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Product: Phospho-DOK2 (Tyr351)[Tyr345] Antibody
Catalog: AF4383
Description: Rabbit polyclonal antibody to Phospho-DOK2 (Tyr351)[Tyr345]
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Dog
Mol.Wt.: 56kDa; 45kD(Calculated).
Uniprot: O60496
RRID: AB_2844448

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(82%), Bovine(92%), Horse(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Phospho-DOK2 (Tyr351) Antibody detects endogenous levels of DOK2 only when phosphorylated at Tyr345, which site historically referenced as Tyr351.
RRID:
AB_2844448
Cite Format: Affinity Biosciences Cat# AF4383, RRID:AB_2844448.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2;

Immunogens

Immunogen:
Uniprot:

O60496(DOK2_HUMAN) >>Visit HPA database.

Gene(ID):
DOK2(9046)
Expression:
O60496 DOK2_HUMAN:

Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

Sequence:
MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
92
Bovine
92
Dog
92
Zebrafish
82
Xenopus
50
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60496 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
Y12 Phosphorylation
S34 Phosphorylation
K49 Ubiquitination
S63 Phosphorylation
S77 Phosphorylation
S122 Phosphorylation
S129 Phosphorylation
Y139 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
T144 Phosphorylation
S169 Phosphorylation
Y170 Phosphorylation
T189 Phosphorylation
Y192 Phosphorylation
S269 Phosphorylation
Y271 Phosphorylation P08631 (HCK) , P07948 (LYN) , P12931 (SRC)
S277 Phosphorylation
S282 Phosphorylation
Y299 Phosphorylation P08631 (HCK) , P07948 (LYN) , P12931 (SRC)
S309 Phosphorylation
K312 Ubiquitination
Y330 Phosphorylation
S332 Phosphorylation
T336 Phosphorylation
Y345 Phosphorylation P07948 (LYN) , P08631 (HCK) , P12931 (SRC)
Y357 Phosphorylation
Y402 Phosphorylation

Research Backgrounds

Function:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).

PTMs:

On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).

Tissue Specificity:

Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

Subunit Structure:

Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).

(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein UL46; this interaction induces DOK2 phosphorylation and subsequent degradation.

Family&Domains:

PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

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