Phospho-IRAK2 (Tyr6) Antibody | Affinity Biosciences

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Product:	Phospho-IRAK2 (Tyr6) Antibody
Catalog:	AF4376
Description:	Rabbit polyclonal antibody to Phospho-IRAK2 (Tyr6)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	62kDa; 69kD(Calculated).
Uniprot:	O43187
RRID:	AB_2844441

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

Phospho-IRAK2 (Tyr6) Antibody detects endogenous levels of IRAK2 only when phosphorylated at Tyr6.

RRID:

AB_2844441
Cite Format: Affinity Biosciences Cat# AF4376, RRID:AB_2844441.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

il1rak2; Interleukin 1 receptor associated kinase 2; Interleukin 1 receptor associated kinase like 2; Interleukin-1 receptor-associated kinase-like 2; IRAK 2; IRAK-2; Irak2; IRAK2_HUMAN; MGC150550;

Immunogens

Immunogen:

Uniprot:

O43187(IRAK2_HUMAN) >>Visit HPA database.

Gene(ID):

IRAK2(3656)

Expression:

O43187 IRAK2_HUMAN:

Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.

Sequence:

O43187 IRAK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MACYIYQLPSWVLDDLCRNMDALSEWDWMEFASYVITDLTQLRKIKSMERVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLRGRETLLPWSGLSEGTGSSSNTPEETDDVDNSSLDASSSMSVAPWAGAATPLLPTENGEGRLRVIVGREADSSSEACVGLEPPQDVTETSWQIEINEAKRKLMENILLYKEEKVDSIELFGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

Chicken

Dog

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43187 As Substrate

O43187

Site	PTM Type	Source
	Phosphorylation	Uniprot
Y4	Phosphorylation	Uniprot
Y6	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
K176	Ubiquitination	Uniprot
K184	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
K350	Ubiquitination	Uniprot
K363	Ubiquitination	Uniprot
S430	Phosphorylation	Uniprot
S434	Phosphorylation	Uniprot
S438	Phosphorylation	Uniprot
K443	Ubiquitination	Uniprot
K452	Ubiquitination	Uniprot
K457	Ubiquitination	Uniprot
K461	Acetylation	Uniprot

Research Backgrounds

O43187_IRAK2_HUMAN

Function:

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.

Tissue Specificity:

Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.

Subunit Structure:

Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1.

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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Phospho-IRAK2 (Tyr6) Antibody - #AF4376