Phospho-eIF4A2 (Tyr49) Antibody - #AF4373

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Product: Phospho-eIF4A2 (Tyr49) Antibody
Catalog: AF4373
Description: Rabbit polyclonal antibody to Phospho-eIF4A2 (Tyr49)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 47kDa; 46kD(Calculated).
Uniprot: Q14240
RRID: AB_2844438

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-eIF4A2 (Tyr49) Antibody detects endogenous levels of eIF4A2 only when phosphorylated at Tyr49.
RRID:
AB_2844438
Cite Format: Affinity Biosciences Cat# AF4373, RRID:AB_2844438.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; eIF 4A II; eIF-4A-II; EIF4A; eIF4A II; eIF4A-II; EIF4A2; EIF4F; Eukaryotic initiation factor 4A II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; Eukaryotic translation initiation factor 4A2; IF4A2_HUMAN; N-terminally processed;

Immunogens

Immunogen:
Uniprot:

Q14240(IF4A2_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4A2(1974)
Sequence:
MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14240 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
Y49 Phosphorylation
Y51 Phosphorylation
K55 Acetylation
K55 Methylation
K55 Sumoylation
K55 Ubiquitination
C67 S-Nitrosylation
K69 Acetylation
K69 Methylation
K69 Ubiquitination
Y71 Phosphorylation
S79 Phosphorylation
T81 Phosphorylation
K83 Ubiquitination
T131 Phosphorylation
K147 Ubiquitination
T159 Phosphorylation
R169 Methylation
K175 Ubiquitination
S190 Phosphorylation
K194 Ubiquitination
Y198 Phosphorylation
T219 Phosphorylation
K226 Sumoylation
K227 Ubiquitination
K239 Ubiquitination
T243 Phosphorylation
K248 Ubiquitination
Y251 Phosphorylation
R256 Methylation
K285 Ubiquitination
K292 Acetylation
K292 Sumoylation
K292 Ubiquitination
K310 Acetylation
K310 Ubiquitination
R317 Methylation
R354 Methylation
K370 Ubiquitination
K382 Ubiquitination

Research Backgrounds

Function:

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Subunit Structure:

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3 (By similarity). Interacts with EIF4E. May interact with NOM1.

(Microbial infection) Interacts with herpes simplex virus 1/HHV-1 protein Vhs.

Family&Domains:

Belongs to the DEAD box helicase family. eIF4A subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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