Product: Phospho-CD79B (Tyr207) Antibody
Catalog: AF4349
Description: Rabbit polyclonal antibody to Phospho-CD79B (Tyr207)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 14kDa, 30-50kDa; 26kD(Calculated).
Uniprot: P40259
RRID: AB_2844428

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CD79B (Tyr207) Antibody detects endogenous levels of CD79B only when phosphorylated at Tyr207.
RRID:
AB_2844428
Cite Format: Affinity Biosciences Cat# AF4349, RRID:AB_2844428.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGM6; B cell antigen receptor complex associated protein beta chain; B cell specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; B29; B29/Ig-beta/CD79b; CD 79b; CD79b; CD79b antigen (immunoglobulin associated beta); CD79b antigen; CD79b molecule; CD79b molecule immunoglobulin associated beta; CD79b protein; CD79B_HUMAN; Ig beta; Ig-beta; IGB; Igbeta; Immunoglobulin associated B29; Immunoglobulin associated B29 protein; Immunoglobulin associated beta; Immunoglobulin associated protein; Immunoglobulin-associated B29 protein; MGC108607;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MARLALSPVPSHWMVALLLLLSAEPVPAARSEDRYRNPKGSACSRIWQSPRFIARKRGFTVKMHCYMNSASGNVSWLWKQEMDENPQQLKLEKGRMEESQNESLATLTIQGIRFEDNGIYFCQQKCNNTSEVYQGCGTELRVMGFSTLAQLKQRNTLKDGIIMIQTLLIILFIIVPIFLLLDKDDSKAGMEEDHTYEGLDIDQTATYEDIVTLRTGEVKWSVGEHPGQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
78
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40259 As Substrate

Site PTM Type Enzyme
S99 Phosphorylation
S146 Phosphorylation
K152 Ubiquitination
T195 Phosphorylation
Y196 Phosphorylation P06241 (FYN) , P51451 (BLK) , P07948 (LYN)
Y207 Phosphorylation P51451 (BLK) , P07948 (LYN) , P06241 (FYN)
K219 Ubiquitination
S221 Phosphorylation

Research Backgrounds

Function:

Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

PTMs:

Phosphorylated on tyrosine upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

B-cells.

Subunit Structure:

Heterodimer of alpha and beta chains; disulfide-linked. Part of the B-cell antigen receptor complex where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin of two heavy chains and two light chains. Interacts with LYN (By similarity).

Research Fields

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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