Phospho-NAKED2 (Tyr247) Antibody | Affinity Biosciences

IF/ICC
pElisa

1/2

AF4347 staining HepG2 cells(4h of LPS treatment) by IF/ICC.

Product:	Phospho-NAKED2 (Tyr247) Antibody
Catalog:	AF4347
Description:	Rabbit polyclonal antibody to Phospho-NAKED2 (Tyr247)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Dog
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	Q969F2
RRID:	AB_2844426

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-NAKED2 (Tyr247) Antibody detects endogenous levels of NAKED2 only when phosphorylated at Tyr247.

RRID:

AB_2844426
Cite Format: Affinity Biosciences Cat# AF4347, RRID:AB_2844426.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hNkd2; Naked 2; Naked-2; nkd2; NKD2_HUMAN; Protein naked cuticle homolog 2;

Immunogens

Immunogen:

Uniprot:

Q969F2(NKD2_HUMAN) >>Visit HPA database.

Gene(ID):

NKD2(85409)

Expression:

Q969F2 NKD2_HUMAN:

Expressed in kidney, lung, pancreas and spleen.

Sequence:

Q969F2 NKD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Zebrafish

100

Bovine

Sheep

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969F2 As Substrate

Q969F2

Site	PTM Type	Source
G2	Myristoylation	Uniprot
S18	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
K74	Ubiquitination	Uniprot
S176	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
T224	Phosphorylation	Uniprot
S230	Phosphorylation	Uniprot
Y247	Phosphorylation	Uniprot
Y256	Phosphorylation	Uniprot
T257	Phosphorylation	Uniprot
S264	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
S299	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot
S394	Phosphorylation	Uniprot
Y423	Phosphorylation	Uniprot

Research Backgrounds

Q969F2_NKD2_HUMAN

Function:

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.

PTMs:

Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.

Subcellular Location:

Cell membrane. Cytoplasm. Cytoplasmic vesicle.

Tissue Specificity:

Expressed in kidney, lung, pancreas and spleen.

Subunit Structure:

Interacts with DVL1, DVL2, DVL3 and PPP2R3A (By similarity). Interacts with RNF25 and TGFA (via cytoplasmic domain).

Family&Domains:

The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.

Belongs to the NKD family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

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Phospho-NAKED2 (Tyr247) Antibody - #AF4347