Product: Phospho-SAR1A (Thr139) Antibody
Catalog: AF4330
Description: Rabbit polyclonal antibody to Phospho-SAR1A (Thr139)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: Q9NR31
RRID: AB_2844409

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Phospho-SAR1A (Thr139) Antibody detects endogenous levels of SAR1A only when phosphorylated at Thr139.
RRID:
AB_2844409
Cite Format: Affinity Biosciences Cat# AF4330, RRID:AB_2844409.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COPII associated small GTPase; COPII-associated small GTPase; GTP binding protein SAR1a; GTP-binding protein SAR1a; Masra 2; Masra2; SAR 1; SAR1 gene homolog A; SAR1 homolog A (S. cerevisiae); SAR1A; SAR1a gene homolog (S cerevisiae) 1; SAR1a gene homolog 1 (S cerevisiae); SAR1a gene homolog 1; SAR1A_HUMAN; SARA 1; Sara; SARA1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
83
Chicken
83
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NR31 As Substrate

Site PTM Type Enzyme
K27 Ubiquitination
T39 Phosphorylation
T40 Phosphorylation
K46 Ubiquitination
T60 Phosphorylation
Y89 Phosphorylation
T120 Phosphorylation
T123 Phosphorylation
S125 Phosphorylation
T139 Phosphorylation
S143 Phosphorylation
K146 Acetylation
K146 Ubiquitination
Y154 Phosphorylation
T164 Phosphorylation
K166 Ubiquitination
K182 Ubiquitination

Research Backgrounds

Function:

Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).

Subcellular Location:

Endoplasmic reticulum. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with B3GAT1.

Family&Domains:

Belongs to the small GTPase superfamily. SAR1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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