Phospho-HPK1 (Ser421) Antibody - #AF4328
| Product: | Phospho-HPK1 (Ser421) Antibody |
| Catalog: | AF4328 |
| Description: | Rabbit polyclonal antibody to Phospho-HPK1 (Ser421) |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Sheep, Rabbit, Dog |
| Mol.Wt.: | 97kDa; 91kD(Calculated). |
| Uniprot: | Q92918 |
| RRID: | AB_2844407 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4328, RRID:AB_2844407.
Fold/Unfold
Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen activated protein kinase kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1;
Immunogens
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
- Q92918 M4K1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92918 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y28 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S171 | Phosphorylation | Q92918 (MAP4K1) , Q15139 (PRKD1) | Uniprot |
| T175 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S258 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S366 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | P43405 (SYK) | Uniprot |
| S405 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| S737 | Phosphorylation | Uniprot |
PTMs - Q92918 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| Q13094 (LCP2) | S376 | Uniprot |
| Q16584 (MAP3K11) | S281 | Uniprot |
| Q92918 (MAP4K1) | T165 | Uniprot |
| Q92918 (MAP4K1) | S171 | Uniprot |
| Q92918 (MAP4K1) | T175 | Uniprot |
| Q92918 (MAP4K1) | T355 | Uniprot |
| Q92918-2 (MAP4K1) | S376 | Uniprot |
| Q92918-2 (MAP4K1) | S377 | Uniprot |
| Q92918-2 (MAP4K1) | Y381 | Uniprot |
| Q92918-2 (MAP4K1) | S407 | Uniprot |
| Q92918-1 (MAP4K1) | S421 | Uniprot |
| Q92918-1 (MAP4K1) | S586 | Uniprot |
| Q9BXL7 (CARD11) | S556 | Uniprot |
| Q9BXL7 (CARD11) | S558 | Uniprot |
Research Backgrounds
Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).
Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Tyrosine-phosphorylated after activation of hemopoietic cells.
Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Interacts with MAP3K1. Interacts with FBXW8. Interacts with CLNK (via its SH2 domain) (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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