Product: Phospho-HPK1 (Ser421) Antibody
Catalog: AF4328
Description: Rabbit polyclonal antibody to Phospho-HPK1 (Ser421)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep, Rabbit, Dog
Mol.Wt.: 97kDa; 91kD(Calculated).
Uniprot: Q92918
RRID: AB_2844407

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-HPK1 (Ser421) Antibody detects endogenous levels of HPK1 only when phosphorylated at Ser421.
RRID:
AB_2844407
Cite Format: Affinity Biosciences Cat# AF4328, RRID:AB_2844407.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen activated protein kinase kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92918 M4K1_HUMAN:

Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.

Sequence:
MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92918 As Substrate

Site PTM Type Enzyme
Y28 Phosphorylation
K33 Ubiquitination
K37 Ubiquitination
K46 Ubiquitination
K49 Ubiquitination
T165 Phosphorylation Q92918 (MAP4K1)
S171 Phosphorylation Q92918 (MAP4K1) , Q15139 (PRKD1)
T175 Phosphorylation Q92918 (MAP4K1)
Y177 Phosphorylation
S230 Phosphorylation
Y232 Phosphorylation P00519 (ABL1)
S258 Phosphorylation
K296 Ubiquitination
S320 Phosphorylation
S324 Phosphorylation
S325 Phosphorylation
S326 Phosphorylation
T349 Phosphorylation
T355 Phosphorylation Q92918 (MAP4K1)
S366 Phosphorylation
S368 Phosphorylation
S374 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
Y381 Phosphorylation P43405 (SYK)
S405 Phosphorylation
S407 Phosphorylation
S413 Phosphorylation
S421 Phosphorylation
S430 Phosphorylation
S436 Phosphorylation
S444 Phosphorylation
S446 Phosphorylation
S447 Phosphorylation
T451 Phosphorylation
S454 Phosphorylation
S586 Phosphorylation
K594 Ubiquitination
S598 Phosphorylation
T599 Phosphorylation
K600 Ubiquitination
S737 Phosphorylation

PTMs - Q92918 As Enzyme

Substrate Site Source
Q13094 (LCP2) S376 Uniprot
Q16584 (MAP3K11) S281 Uniprot
Q92918 (MAP4K1) T165 Uniprot
Q92918 (MAP4K1) S171 Uniprot
Q92918 (MAP4K1) T175 Uniprot
Q92918 (MAP4K1) T355 Uniprot
Q92918-2 (MAP4K1) S376 Uniprot
Q92918-2 (MAP4K1) S377 Uniprot
Q92918-2 (MAP4K1) Y381 Uniprot
Q92918-2 (MAP4K1) S407 Uniprot
Q92918-1 (MAP4K1) S421 Uniprot
Q92918-1 (MAP4K1) S586 Uniprot
Q9BXL7 (CARD11) S556 Uniprot
Q9BXL7 (CARD11) S558 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).

PTMs:

Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.

Tyrosine-phosphorylated after activation of hemopoietic cells.

Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.

Tissue Specificity:

Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.

Subunit Structure:

Interacts with MAP3K1. Interacts with FBXW8. Interacts with CLNK (via its SH2 domain) (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.