Phospho-SMURF1 (Ser200) Antibody | Affinity Biosciences

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Product:	Phospho-SMURF1 (Ser200) Antibody
Catalog:	AF4323
Description:	Rabbit polyclonal antibody to Phospho-SMURF1 (Ser200)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	55-90kD; 86kD(Calculated).
Uniprot:	Q9HCE7
RRID:	AB_2844402

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-SMURF1 (Ser200) Antibody detects endogenous levels of SMURF1 only when phosphorylated at Ser200.

RRID:

AB_2844402
Cite Format: Affinity Biosciences Cat# AF4323, RRID:AB_2844402.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin-protein ligase SMURF1; hSMURF1; KIAA1625; Smad specific E3 ubiquitin ligase 1; SMAD specific E3 ubiquitin protein ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMUF1_HUMAN; SMURF 1; smurf1;

Immunogens

Immunogen:

Uniprot:

Q9HCE7(SMUF1_HUMAN) >>Visit HPA database.

Gene(ID):

SMURF1(57154)

Expression:

Q9HCE7 SMUF1_HUMAN:

Expressed in melanocytes (PubMed:23999003).

Sequence:

Q9HCE7 SMUF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCE7 As Substrate

Q9HCE7

Site	PTM Type	Source
Y66	Phosphorylation	Uniprot
Y69	Phosphorylation	Uniprot
T145	Phosphorylation	Uniprot
S148	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
Y251	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot
T332	Phosphorylation	Uniprot
K350	Ubiquitination	Uniprot
K381	Ubiquitination	Uniprot
K383	Ubiquitination	Uniprot
S412	Phosphorylation	Uniprot
Y413	Phosphorylation	Uniprot
K418	Ubiquitination	Uniprot
Y440	Phosphorylation	Uniprot
K521	Ubiquitination	Uniprot
S656	Phosphorylation	Uniprot
K739	Ubiquitination	Uniprot

Research Backgrounds

Q9HCE7_SMUF1_HUMAN

Function:

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.

PTMs:

Auto-ubiquitinated in presence of NDFIP1. Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Expressed in melanocytes.

Subunit Structure:

Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. Interacts with MAVS; the interaction is mediated by NDFIP1.

Family&Domains:

The C2 domain mediates membrane localization and substrate selection.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

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Phospho-SMURF1 (Ser200) Antibody - #AF4323