Phospho-GRAP2 (Thr262) Antibody - #AF4322

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Product: Phospho-GRAP2 (Thr262) Antibody
Catalog: AF4322
Description: Rabbit polyclonal antibody to Phospho-GRAP2 (Thr262)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 25,38 kDa; 38kD(Calculated).
Uniprot: O75791
RRID: AB_2844401

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-GRAP2 (Thr262) Antibody detects endogenous levels of GRAP2 only when phosphorylated at Thr262.
RRID:
AB_2844401
Cite Format: Affinity Biosciences Cat# AF4322, RRID:AB_2844401.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter protein GRID; GADS; GADS protein; GRAP-2; Grap2; GRAP2_HUMAN; GRB-2-like protein; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRB2L; GRBLG; GRBX; Grf-40; Grf40 adapter protein; Grf40; GRID; Growth factor receptor-binding protein; Growth factor receptor-bound protein 2-related adaptor protein 2; GRPL; Hema; Hematopoietic cell-associated adapter protein GrpL; Hematopoietic cell-associated adaptor protein GRPL; Mona; p38; Protein GADS; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule;

Immunogens

Immunogen:
Uniprot:

O75791(GRAP2_HUMAN) >>Visit HPA database.

Gene(ID):
GRAP2(9402)
Sequence:
MEAVAKFDFTASGEDELSFHTGDVLKILSNQEEWFKAELGSQEGYVPKNFIDIQFPKWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSISRQKQIFLRDRTREDQGHRGNSLDRRSQGGPHLSGAVGEEIRPSMNRKLSDHPPTLPLQQHQHQPQPPQYAPAPQQLQQPPQQRYLQHHHFHQERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALYDFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75791 As Substrate

Site PTM Type Enzyme
K36 Ubiquitination
S41 Phosphorylation
Y45 Phosphorylation
K48 Ubiquitination
K57 Ubiquitination
K106 Acetylation
K112 Ubiquitination
K121 Ubiquitination
K127 Ubiquitination
Y131 Phosphorylation
K141 Ubiquitination
S159 Phosphorylation
S164 Phosphorylation
S181 Phosphorylation
S187 Phosphorylation
T192 Phosphorylation
Y207 Phosphorylation
Y222 Phosphorylation
R233 Methylation
S236 Phosphorylation
T246 Phosphorylation
S250 Phosphorylation
T262 Phosphorylation
Y324 Phosphorylation

Research Backgrounds

Function:

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Subcellular Location:

Nucleus. Cytoplasm. Endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with phosphorylated LIME1 upon TCR activation (By similarity). Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23.

Family&Domains:

Belongs to the GRB2/sem-5/DRK family.

Research Fields

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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