Phospho-DEPTOR (Ser244) Antibody - #AF4313

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Product: Phospho-DEPTOR (Ser244) Antibody
Catalog: AF4313
Description: Rabbit polyclonal antibody to Phospho-DEPTOR (Ser244)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 48kDa; 46kD(Calculated).
Uniprot: Q8TB45
RRID: AB_2844392

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(90%), Bovine(100%), Horse(100%), Rabbit(91%), Dog(100%), Chicken(80%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
Phospho-DEPTOR (Ser244) Antibody detects endogenous levels of DEPTOR only when phosphorylated at Ser244.
RRID:
AB_2844392
Cite Format: Affinity Biosciences Cat# AF4313, RRID:AB_2844392.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DEP domain containing 6; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPDC6; DEPTOR; DPTOR_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8TB45(DPTOR_HUMAN) >>Visit HPA database.

Gene(ID):
DEPTOR(64798)
Sequence:
MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSNKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
91
Zebrafish
90
Xenopus
82
Chicken
80
Pig
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TB45 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S6 Phosphorylation
T7 Phosphorylation
S9 Phosphorylation
K58 Ubiquitination
T59 Phosphorylation
K91 Ubiquitination
K131 Ubiquitination
K142 Ubiquitination
S145 Phosphorylation
T149 Phosphorylation P48729 (CSNK1A1)
K160 Ubiquitination
S168 Phosphorylation
K234 Ubiquitination
S235 Phosphorylation
S237 Phosphorylation
T241 Phosphorylation
S244 Phosphorylation
S258 Phosphorylation
T259 Phosphorylation
S260 Phosphorylation
S263 Phosphorylation P48729 (CSNK1A1)
S265 Phosphorylation P42345 (MTOR)
S267 Phosphorylation
S279 Phosphorylation
S280 Phosphorylation
S282 Phosphorylation
S283 Phosphorylation
S286 Phosphorylation P48729 (CSNK1A1) , Q15418 (RPS6KA1) , P42345 (MTOR) , P23443 (RPS6KB1)
S287 Phosphorylation Q15418 (RPS6KA1) , P48729 (CSNK1A1) , P23443 (RPS6KB1)
S291 Phosphorylation P48729 (CSNK1A1) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
S292 Phosphorylation
S293 Phosphorylation P42345 (MTOR)
T295 Phosphorylation P42345 (MTOR)
S297 Phosphorylation
S298 Phosphorylation
S299 Phosphorylation P42345 (MTOR)
K311 Ubiquitination
T315 Phosphorylation
T321 Phosphorylation
K348 Ubiquitination
T387 Phosphorylation
S389 Phosphorylation
T394 Phosphorylation

Research Backgrounds

Function:

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.

PTMs:

Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.

Ubiquitinated; ubiquitination leads to proteasomal degradation.

Subunit Structure:

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2. Interacts (via PDZ domain) with MINAR1 (via N-terminus). Interacts with SIK3.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

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