Phospho-MRIP (Thr542) Antibody - #AF4308

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Product: Phospho-MRIP (Thr542) Antibody
Catalog: AF4308
Description: Rabbit polyclonal antibody to Phospho-MRIP (Thr542)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 115kDa,150kDa; 117kD(Calculated).
Uniprot: Q6WCQ1
RRID: AB_2844387

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MRIP (Thr542) Antibody detects endogenous levels of MRIP only when phosphorylated at Thr542.
RRID:
AB_2844387
Cite Format: Affinity Biosciences Cat# AF4308, RRID:AB_2844387.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9530046C02; AA536749; AI647711; C76423; KIAA0864; M-RIP; MGC67316; mKIAA0864; Mprip; MPRIP_HUMAN; Myosin phosphatase Rho-interacting protein; p116 Rho-interacting protein; p116Rip; Rho-interacting protein 3; Rhoip3; RIP3; RP23-180B18.4;

Immunogens

Immunogen:
Uniprot:

Q6WCQ1(MPRIP_HUMAN) >>Visit HPA database.

Gene(ID):
MPRIP(23164)
Sequence:
MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQRRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSGWLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRTKDQPDGSSLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAEEQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTRQGRSEKRAFPRKRDFTNEAPPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVGPADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTHNVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLLQDQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSVIEQVSWDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6WCQ1 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
T166 Phosphorylation
S181 Phosphorylation
S182 Phosphorylation
S183 Phosphorylation
S184 Phosphorylation
S192 Phosphorylation
T210 Phosphorylation
S217 Phosphorylation
S218 Phosphorylation
S220 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
S228 Phosphorylation
S265 Phosphorylation
Y267 Phosphorylation
S269 Phosphorylation
S289 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
T295 Phosphorylation
S301 Phosphorylation
K306 Ubiquitination
S326 Phosphorylation
S329 Phosphorylation
T348 Phosphorylation
S360 Phosphorylation
S362 Phosphorylation
S365 Phosphorylation
S372 Phosphorylation
S377 Phosphorylation
T378 Phosphorylation
T383 Phosphorylation
K390 Ubiquitination
K396 Ubiquitination
Y450 Phosphorylation
S493 Phosphorylation
S501 Phosphorylation
S504 Phosphorylation
K527 Ubiquitination
S540 Phosphorylation
T542 Phosphorylation
T602 Phosphorylation
S619 Phosphorylation
S625 Phosphorylation
T646 Phosphorylation
S662 Phosphorylation
S663 Phosphorylation
K681 Ubiquitination
K688 Ubiquitination
S691 Phosphorylation
S713 Phosphorylation
Y718 Phosphorylation
S800 Phosphorylation
S815 Phosphorylation
S891 Phosphorylation
Y915 Phosphorylation
K917 Ubiquitination
S921 Phosphorylation
Y945 Phosphorylation
T946 Phosphorylation
T970 Phosphorylation
K976 Ubiquitination
S977 Phosphorylation
S980 Phosphorylation
S984 Phosphorylation
Y986 Phosphorylation
K990 Methylation
S991 Phosphorylation
K992 Methylation
S993 Phosphorylation
S1014 Phosphorylation
S1016 Phosphorylation

Research Backgrounds

Function:

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Colocalizes with F-actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds F-actin through its N-terminus. Interacts with MYZAP (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.

Restrictive clause

 

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For Research Use Only.
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