Phospho-DKK1 (Ser140) Antibody | Affinity Biosciences

WB
pElisa

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Western blot analysis of extracts from various samples, using Phospho-DKK1 (Ser140) Antibody.

Western blot analysis of extracts from MCF7 cells(serum starvation treatment), using Phospho-DKK1 (Ser140) Antibody.

Product:	Phospho-DKK1 (Ser140) Antibody
Catalog:	AF4300
Description:	Rabbit polyclonal antibody to Phospho-DKK1 (Ser140)
Application:	WB
Reactivity:	Human, Mouse
Mol.Wt.:	28-40kDa; 29kD(Calculated).
Uniprot:	O94907
RRID:	AB_2844379

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-DKK1 (Ser140) Antibody detects endogenous levels of DKK1 only when phosphorylated at Ser140.

RRID:

AB_2844379
Cite Format: Affinity Biosciences Cat# AF4300, RRID:AB_2844379.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dickkopf 1; Dickkopf 1 homolog; Dickkopf 1 like; Dickkopf homolog 1; Dickkopf like protein 1; Dickkopf related protein 1; Dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; Dickkopf-related protein 1; DKK 1; Dkk-1; Dkk1; DKK1_HUMAN; hDkk 1; hDkk-1; SK;

Immunogens

Immunogen:

Uniprot:

O94907(DKK1_HUMAN) >>Visit HPA database.

Gene(ID):

DKK1(22943)

Expression:

O94907 DKK1_HUMAN:

Placenta.

Sequence:

O94907 DKK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMALGAAGATRVFVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH

PTMs - O94907 As Substrate

O94907

Site	PTM Type	Source
S61	O-Glycosylation	Uniprot
Y132	Phosphorylation	Uniprot
S140	Phosphorylation	Uniprot
S141	Phosphorylation	Uniprot
T155	O-Glycosylation	Uniprot
S163	O-Glycosylation	Uniprot
T164	O-Glycosylation	Uniprot
S169	O-Glycosylation	Uniprot
T172	O-Glycosylation	Uniprot
T173	O-Glycosylation	Uniprot
T181	O-Glycosylation	Uniprot
T221	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
N256	N-Glycosylation	Uniprot
S257	Phosphorylation	Uniprot
S258	Phosphorylation	Uniprot
T262	Phosphorylation	Uniprot

Research Backgrounds

O94907_DKK1_HUMAN

Function:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

Subcellular Location:

Secreted.

Tissue Specificity:

Placenta.

Subunit Structure:

Interacts with LRP6. Interacts (via the C-termianl Cys-rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt-mediated signaling. Forms a ternary complex with LRP6 and KREM1. Interacts with KREM1.

Family&Domains:

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

Belongs to the dickkopf family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

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Phospho-DKK1 (Ser140) Antibody - #AF4300