Mre11 Antibody - #DF10364

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Product: Mre11 Antibody
Catalog: DF10364
Description: Rabbit polyclonal antibody to Mre11
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 80kDa; 81kD(Calculated).
Uniprot: P49959
RRID: AB_2844371

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Mre11 Antibody detects endogenous levels of total Mre11.
RRID:
AB_2844371
Cite Format: Affinity Biosciences Cat# DF10364, RRID:AB_2844371.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AT like disease; Ataxia telangiectasia disorder like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; Meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A (S. cerevisiae); Meiotic recombination 11 homolog A; MmMRE11A; Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A; MRE11_HUMAN; MRE11A; MRE11b; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833;

Immunogens

Immunogen:
Uniprot:

P49959(MRE11_HUMAN) >>Visit HPA database.

Gene(ID):
MRE11(4361)
Sequence:
MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHIDALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR

PTMs - P49959 As Substrate

Site PTM Type Enzyme
S2 Acetylation
T12 Phosphorylation
K70 Ubiquitination
T74 Phosphorylation
S158 Phosphorylation
K161 Ubiquitination
S165 Phosphorylation
K171 Ubiquitination
S183 Phosphorylation
Y190 Phosphorylation
K196 Acetylation
K197 Acetylation
K197 Ubiquitination
K255 Ubiquitination
S264 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
T272 Phosphorylation
S273 Phosphorylation
S275 Phosphorylation
K282 Ubiquitination
K298 Ubiquitination
T329 Phosphorylation
K339 Ubiquitination
K360 Ubiquitination
S382 Phosphorylation Q13535 (ATR)
K384 Ubiquitination
R388 Methylation
K393 Ubiquitination
K407 Ubiquitination
K416 Acetylation
K416 Ubiquitination
K420 Ubiquitination
T426 Phosphorylation
K434 Ubiquitination
Y436 Phosphorylation
K442 Ubiquitination
S447 Phosphorylation
K464 Ubiquitination
K475 Ubiquitination
K480 Acetylation
K480 Ubiquitination
T481 Phosphorylation
K486 Ubiquitination
K496 Ubiquitination
S531 Phosphorylation Q13535 (ATR)
S558 Phosphorylation
S561 Phosphorylation
R570 Methylation
R572 Methylation
R574 Methylation
R576 Methylation
R577 Methylation
R580 Methylation
R587 Methylation
S590 Phosphorylation
R592 Methylation
R594 Methylation
T597 Phosphorylation
R604 Methylation
S613 Phosphorylation
R616 Methylation
S619 Phosphorylation
K625 Sumoylation
S649 Phosphorylation P68400 (CSNK2A1)
S667 Phosphorylation
S668 Phosphorylation
T669 Phosphorylation
S671 Phosphorylation
S672 Phosphorylation
K673 Ubiquitination
S676 Phosphorylation Q13315 (ATM)
S678 Phosphorylation Q13315 (ATM)
S681 Phosphorylation
S688 Phosphorylation
S689 Phosphorylation
S701 Phosphorylation
S702 Phosphorylation

Research Backgrounds

Function:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

PTMs:

Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.

Subcellular Location:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity. Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain).

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

Belongs to the MRE11/RAD32 family.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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