eIF2B epsilon Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from H2O2 treated MDA-MB-231 cells, using eIF2B epsilon Antibody.

Product:	eIF2B epsilon Antibody
Catalog:	DF10348
Description:	Rabbit polyclonal antibody to eIF2B epsilon
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	80KD; 80kD(Calculated).
Uniprot:	Q13144
RRID:	AB_2844355

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

eIF2B epsilon Antibody detects endogenous levels of total eIF2B epsilon.

RRID:

AB_2844355
Cite Format: Affinity Biosciences Cat# DF10348, RRID:AB_2844355.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon;

Immunogens

Immunogen:

Uniprot:

Q13144(EI2BE_HUMAN) >>Visit HPA database.

Gene(ID):

EIF2B5(8893)

Sequence:

Q13144 EI2BE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD

PTMs - Q13144 As Substrate

Q13144

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S20	Phosphorylation		Uniprot
S27	Phosphorylation		Uniprot
S51	Phosphorylation	P48729 (CSNK1A1)	Uniprot
K61	Ubiquitination		Uniprot
K103	Ubiquitination		Uniprot
K108	Ubiquitination		Uniprot
K141	Ubiquitination		Uniprot
T182	Phosphorylation		Uniprot
K186	Ubiquitination		Uniprot
K217	Ubiquitination		Uniprot
Y319	Phosphorylation		Uniprot
K420	Ubiquitination		Uniprot
S447	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
S466	Phosphorylation	P48729 (CSNK1A1)	Uniprot
S469	Phosphorylation	P48729 (CSNK1A1)	Uniprot
K481	Ubiquitination		Uniprot
Y483	Phosphorylation		Uniprot
K493	Ubiquitination		Uniprot
S525	Phosphorylation		Uniprot
S527	Phosphorylation		Uniprot
S529	Phosphorylation		Uniprot
S532	Phosphorylation		Uniprot
S535	Phosphorylation	P49841 (GSK3B)	Uniprot
S540	Phosphorylation	P49841 (GSK3B) , P49840 (GSK3A)	Uniprot
S544	Phosphorylation	P49840 (GSK3A) , P49841 (GSK3B)	Uniprot
K565	Ubiquitination		Uniprot
Y583	Phosphorylation		Uniprot
S610	Phosphorylation		Uniprot
C618	S-Nitrosylation		Uniprot
K693	Ubiquitination		Uniprot
S717	Phosphorylation	P68400 (CSNK2A1) , P19784 (CSNK2A2)	Uniprot
S718	Phosphorylation	P68400 (CSNK2A1) , P19784 (CSNK2A2)	Uniprot

Research Backgrounds

Q13144_EI2BE_HUMAN

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

PTMs:

Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.

Polyubiquitinated, probably by NEDD4.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.

Family&Domains:

Belongs to the eIF-2B gamma/epsilon subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

eIF2B epsilon Antibody - #DF10348