eIF2B epsilon Antibody - #DF10346

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Product: eIF2B epsilon Antibody
Catalog: DF10346
Description: Rabbit polyclonal antibody to eIF2B epsilon
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 80KD; 80kD(Calculated).
Uniprot: Q13144
RRID: AB_2844353

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
eIF2B epsilon Antibody detects endogenous levels of total eIF2B epsilon.
RRID:
AB_2844353
Cite Format: Affinity Biosciences Cat# DF10346, RRID:AB_2844353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon;

Immunogens

Immunogen:
Uniprot:

Q13144(EI2BE_HUMAN) >>Visit HPA database.

Gene(ID):
EIF2B5(8893)
Sequence:
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Bovine
88
Sheep
88
Dog
88
Rabbit
75
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13144 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S20 Phosphorylation
S27 Phosphorylation
S51 Phosphorylation P48729 (CSNK1A1)
K61 Ubiquitination
K103 Ubiquitination
K108 Ubiquitination
K141 Ubiquitination
T182 Phosphorylation
K186 Ubiquitination
K217 Ubiquitination
Y319 Phosphorylation
K420 Ubiquitination
S447 Phosphorylation
S450 Phosphorylation
S466 Phosphorylation P48729 (CSNK1A1)
S469 Phosphorylation P48729 (CSNK1A1)
K481 Ubiquitination
Y483 Phosphorylation
K493 Ubiquitination
S525 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S532 Phosphorylation
S535 Phosphorylation P49841 (GSK3B)
S540 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)
S544 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
K565 Ubiquitination
Y583 Phosphorylation
S610 Phosphorylation
C618 S-Nitrosylation
K693 Ubiquitination
S717 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S718 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

Research Backgrounds

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

PTMs:

Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.

Polyubiquitinated, probably by NEDD4.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.

Family&Domains:

Belongs to the eIF-2B gamma/epsilon subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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