NDR1/2 Antibody - #DF10339

(3)  
Product: NDR1/2 Antibody
Catalog: DF10339
Description: Rabbit polyclonal antibody to NDR1/2
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 54KD; 54kD(Calculated).
Uniprot: Q15208 | Q9Y2H1
RRID: AB_2844346

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NDR1/2 Antibody detects endogenous levels of total NDR1/2.
RRID:
AB_2844346
Cite Format: Affinity Biosciences Cat# DF10339, RRID:AB_2844346.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NDR 1; NDR; Ndr Ser/Thr kinase-like protein; NDR1; NDR1 protein kinase; Nuclear Dbf2 related kinase 1; nuclear Dbf2-related 1; Nuclear Dbf2-related kinase 1; Nuclear Dbf2-related protein; Serine/threonine protein kinase 38; Serine/threonine-protein kinase 38; STK 38; STK38; STK38_HUMAN; KIAA0965; NDR2; NDR2 protein kinase; nuclear Dbf2 related 2; Nuclear Dbf2 related kinase 2; Nuclear Dbf2-related kinase 2; Serine/threonine protein kinase 38 like; Serine/threonine-protein kinase 38-like; ST38L_HUMAN; Stk38l;

Immunogens

Immunogen:
Uniprot:

Q15208(STK38_HUMAN) >>Visit HPA database.

Q9Y2H1(ST38L_HUMAN) >>Visit HPA database.

Gene(ID):
STK38L(23012) | STK38(11329)
Expression:
Q15208 STK38_HUMAN:

Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.

Q9Y2H1 ST38L_HUMAN:

Ubiquitously expressed with highest levels observed in the thymus.

Sequence:
MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

MAMTAGTTTTFPMSNHTRERVTVAKLTLENFYSNLILQHEERETRQKKLEVAMEEEGLADEEKKLRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHIYAMKILRKSDMLEKEQVAHIRAERDILVEADGAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYISETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGLCTGLKKAHRTEFYRNLTHNPPSDFSFQNMNSKRKAETWKKNRRQLAYSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWKETLVFPPEVPISEKAKDLILRFCIDSENRIGNSGVEEIKGHPFFEGVDWEHIRERPAAIPIEIKSIDDTSNFDDFPESDILQPVPNTTEPDYKSKDWVFLNYTYKRFEGLTQRGSIPTYMKAGKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15208/Q9Y2H1 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
S6 Phosphorylation P49841 (GSK3B)
T7 Phosphorylation P49841 (GSK3B)
S10 Phosphorylation P17612 (PRKACA)
S11 Phosphorylation P17612 (PRKACA)
S13 Phosphorylation
T16 Phosphorylation
K17 Ubiquitination
T26 Phosphorylation
Y31 Phosphorylation
K58 Acetylation
K62 Ubiquitination
K72 Ubiquitination
T74 Phosphorylation Q15208 (STK38)
K93 Ubiquitination
K109 Ubiquitination
K118 Ubiquitination
K122 Ubiquitination
K128 Ubiquitination
T183 Phosphorylation
K214 Ubiquitination
K227 Ubiquitination
C234 S-Nitrosylation
T235 Phosphorylation
K238 Ubiquitination
S252 Phosphorylation
S264 Phosphorylation
K265 Ubiquitination
T270 Phosphorylation
S281 Phosphorylation Q15208 (STK38)
T282 Phosphorylation
T285 Phosphorylation
Y288 Phosphorylation
K338 Ubiquitination
K354 Ubiquitination
K377 Ubiquitination
T407 Phosphorylation
K420 Ubiquitination
K434 Ubiquitination
Y443 Phosphorylation
T444 Phosphorylation P26927 (MST1) , Q15208 (STK38) , Q9Y6E0 (STK24) , Q13043 (STK4)
Y445 Phosphorylation
T452 Phosphorylation
R454 Methylation
Y460 Phosphorylation
K462 Ubiquitination
Site PTM Type Enzyme
K73 Ubiquitination
T75 Phosphorylation
K94 Ubiquitination
K110 Ubiquitination
Y116 Phosphorylation
K119 Ubiquitination
K123 Ubiquitination
K129 Ubiquitination
K215 Ubiquitination
K224 Ubiquitination
K228 Ubiquitination
T236 Phosphorylation
K239 Ubiquitination
S265 Phosphorylation
K266 Ubiquitination
S282 Phosphorylation Q9Y2H1 (STK38L)
T283 Phosphorylation
T286 Phosphorylation
Y289 Phosphorylation
K355 Ubiquitination
K378 Ubiquitination
K403 Ubiquitination
Y441 Phosphorylation
T442 Phosphorylation Q9Y6E0 (STK24)
Y443 Phosphorylation
T450 Phosphorylation
R452 Methylation
S454 Phosphorylation
K460 Ubiquitination

PTMs - Q15208/Q9Y2H1 As Enzyme

Substrate Site Source
P38936 (CDKN1A) S146 Uniprot
P46937 (YAP1) S61 Uniprot
P46937 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937 (YAP1) S164 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q15208 (STK38) T74 Uniprot
Q15208 (STK38) S281 Uniprot
Q15208 (STK38) T444 Uniprot
Q2M2I8 (AAK1) S637 Uniprot
Q96QF0 (RAB3IP) S288 Uniprot
Q96RD6 (PANX2) S514 Uniprot
Q9BXF6 (RAB11FIP5) S307 Uniprot
Q9UBF8 (PI4KB) S277 Uniprot
Substrate Site Source
O60285 (NUAK1) T211 Uniprot
P46937 (YAP1) S127 Uniprot
Q96QF0 (RAB3IP) S288 Uniprot
Q9Y2H1 (STK38L) S282 Uniprot

Research Backgrounds

Function:

Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.

PTMs:

ISGylated.

Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.

Subunit Structure:

Homodimeric S100B binds two molecules of STK38. Interacts with MOB1 and MOB2. Interacts with MAP3K1 and MAP3K2 (via the kinase catalytic domain). Forms a tripartite complex with MOBKL1B and STK3/MST2. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Function:

Involved in the regulation of structural processes in differentiating and mature neuronal cells.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Membrane.
Note: Associated with the actin cytoskeleton. Co-localizes with STK24/MST3 in the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels observed in the thymus.

Subunit Structure:

Homodimeric S100B binds two molecules of STK38L. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity). Interacts with MOB1 and MOB2.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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