VAMP4 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of VAMP4 in lysates of K562 , using VAMP4 Antibody(DF10325).

Product:	VAMP4 Antibody
Catalog:	DF10325
Description:	Rabbit polyclonal antibody to VAMP4
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	16KD; 16kD(Calculated).
Uniprot:	O75379
RRID:	AB_2844332

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

VAMP4 Antibody detects endogenous levels of total VAMP4.

RRID:

AB_2844332
Cite Format: Affinity Biosciences Cat# DF10325, RRID:AB_2844332.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

VAMP 24; VAMP 4; VAMP-4; VAMP24; VAMP4; VAMP4 protein; VAMP4_HUMAN; Vesicle associated membrane protein 4; Vesicle associated membrane protein 4 isoform CRA a; Vesicle associated membrane protein 4 isoform CRA b; Vesicle-associated membrane protein 4;

Immunogens

Immunogen:

Uniprot:

O75379(VAMP4_HUMAN) >>Visit HPA database.

Gene(ID):

VAMP4(8674)

Sequence:

O75379 VAMP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75379 As Substrate

O75379

Site	PTM Type	Enzyme	Source
S17	Phosphorylation		Uniprot
K19	Ubiquitination		Uniprot
S30	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K73	Ubiquitination		Uniprot
K87	Ubiquitination		Uniprot
S88	Phosphorylation		Uniprot
S90	Phosphorylation		Uniprot
S92	Phosphorylation		Uniprot
Y139	Phosphorylation		Uniprot

Research Backgrounds

O75379_VAMP4_HUMAN

Function:

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Single-pass type IV membrane protein.
Note: Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.

Subunit Structure:

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity). Interacts with BAIAP3; this interaction is increased in the presence of calcium.

Family&Domains:

Belongs to the synaptobrevin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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VAMP4 Antibody - #DF10325