TRPV1 Antibody | Affinity Biosciences

IF/ICC
Cites

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DF10320 staining Hela cells by IF/ICC.

FIGURE 8 Bufalin-PLGA MS suppressed the protein expression of TRPV1 and P2X7 in CCI rats.

FIGURE 9 Expressions of TRPV1 and P2X7 were visualized and quantified via immunofluorescence staining.

Fig.

Product:	TRPV1 Antibody
Catalog:	DF10320
Description:	Rabbit polyclonal antibody to TRPV1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	95kDa; 95kD(Calculated).
Uniprot:	Q8NER1
RRID:	AB_2844327

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(83%)

Clonality:

Polyclonal

Specificity:

VR1 Antibody detects endogenous levels of total VR1.

RRID:

AB_2844327
Cite Format: Affinity Biosciences Cat# DF10320, RRID:AB_2844327.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Capsaicin receptor; DKFZp434K0220; osm 9 like TRP channel 1; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; TRPV 1; Trpv1; TRPV1_HUMAN; Vanilloid receptor 1; Vanilloid receptor subtype 1; VR 1; VR1;

Immunogens

Immunogen:

Uniprot:

Q8NER1(TRPV1_HUMAN) >>Visit HPA database.

Gene(ID):

TRPV1(7442)

Expression:

Q8NER1 TRPV1_HUMAN:

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

Sequence:

Q8NER1 TRPV1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTADNTKFVTSMYNEILMLGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKTGDYFRVTGEILSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NER1 As Substrate

Q8NER1

Site	PTM Type	Enzyme	Source
S5	Phosphorylation		Uniprot
S6	Phosphorylation		Uniprot
T7	Phosphorylation		Uniprot
S117	Phosphorylation	Q15139 (PRKD1)	Uniprot
S132	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
K161	Acetylation		Uniprot
T196	Phosphorylation		Uniprot
Y200	Phosphorylation	P12931 (SRC)	Uniprot
T336	Phosphorylation		Uniprot
T407	Phosphorylation		Uniprot
S502	Phosphorylation	Q02156 (PRKCE) , P17612 (PRKACA) , P17252 (PRKCA)	Uniprot
T705	Phosphorylation	P05771-2 (PRKCB)	Uniprot
T773	Phosphorylation		Uniprot
S775	Phosphorylation		Uniprot
S780	Phosphorylation		Uniprot
S801	Phosphorylation	Q02156 (PRKCE) , P17252 (PRKCA)	Uniprot
S807	Phosphorylation		Uniprot
Y814	Phosphorylation		Uniprot

Research Backgrounds

Q8NER1_TRPV1_HUMAN

Function:

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.

PTMs:

Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell projection>Dendritic spine membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Mostly, but not exclusively expressed in postsynaptic dendritic spines.

Tissue Specificity:

Subunit Structure:

Interacts with PIRT (By similarity). Homotetramer (By similarity). Interacts with TRPV3 and may also form a heteromeric channel with TRPV3. Interacts with CALM, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity). Interacts with the Scolopendra mutilans RhTx toxin (By similarity). Interacts with TMEM100 (By similarity). Interacts with PACS2.

Family&Domains:

The association domain (AD) is necessary for self-association.

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

References

1). A quinoa peptide protects impaired mucus barriers in colitis mice by inhibiting NF-κB-TRPV1 signaling and regulating the gut microbiota. Food & function, 2024 (PubMed: 38226896) [IF=6.1]

2). Selective activation of AKAP150/TRPV1 in ventrolateral periaqueductal gray GABAergic neurons facilitates conditioned place aversion in male mice. Communications Biology, 2023 (PubMed: 37460788) [IF=5.9]

Application: WB Species: Mouse Sample:

Fig. 5 TRPV1 in GABAergic neurons of the vlPAG in CPA mice. Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P

3). Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model. Frontiers in Pharmacology, 2022 (PubMed: 35770074) [IF=5.6]

Application: WB Species: Rat Sample:

FIGURE 8 Bufalin-PLGA MS suppressed the protein expression of TRPV1 and P2X7 in CCI rats. (A) SDS-PAGE bands of TRPV1 and P2X7 protein in the DRG of different group rats. (B) Expression levels of TRPV1 protein in the DRG of different group rats. (C) Expression level of P2X7 protein in the DRG of different group rats. Data are expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group.

Application: IF/ICC Species: Rat Sample:

FIGURE 9 Expressions of TRPV1 and P2X7 were visualized and quantified via immunofluorescence staining. (A) Immunofluorescence detection of TRPV1 protein in the DRG; green and blue staining represent TRPV1 and DAPI, respectively. (B) Immunofluorescence detection of P2X7 protein in the DRG; green and blue staining represent P2X7 and DAPI, respectively. Data is expressed in the form of mean ± SEM, n = 3. ###p < 0.001 compared to the control group, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the CCI group. (Scale bar = 20 µm).

4). Kemin capsule ameliorates post-infectious cough by modulating the PI3K/AKT signaling pathway and TRPA1/TRPV1 channels. JOURNAL OF ETHNOPHARMACOLOGY, 2024 [IF=4.8]

5). Capsaicin exerts synergistic pro-apoptotic effects with cisplatin in TSCC through the calpain pathway via TRPV1. Journal of Cancer, 2024 (PubMed: 39132151) [IF=3.9]

6). Transient receptor potential vanilloid type-1 regulates periodontal disease damage via the PI3K/AKT signaling pathway. Iranian Journal of Basic Medical Sciences, 2022 (PubMed: 35911651) [IF=2.2]

7). Capsaicin exerts anti-benign prostatic hyperplasia effects via inhibiting androgen receptor signaling pathway.. Biocell, 2021 [IF=1.2]

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TRPV1 Antibody - #DF10320