Kv3.4/KCNC4 Antibody | Affinity Biosciences

WB
Cites

1/2

Western blot analysis of extracts from Mouse brain tissue, using Kv3.

FIGURE 3 | Protein expression of α2NKA, α1NKA, and Kv3.

Product:	Kv3.4/KCNC4 Antibody
Catalog:	DF10312
Description:	Rabbit polyclonal antibody to Kv3.4/KCNC4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	70KD; 70kD(Calculated).
Uniprot:	Q03721
RRID:	AB_2844319

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Kv3.4/KCNC4 Antibody detects endogenous levels of total Kv3.4/KCNC4.

RRID:

AB_2844319
Cite Format: Affinity Biosciences Cat# DF10312, RRID:AB_2844319.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q03721(KCNC4_HUMAN) >>Visit HPA database.

Gene(ID):

KCNC4(3749)

Sequence:

Q03721 KCNC4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MISSVCVSSYRGRKSGNKPPSKTCLKEEMAKGEASEKIIINVGGTRHETYRSTLRTLPGTRLAWLADPDGGGRPETDGGGVGSSGSSGGGGCEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELTFWGIDETDVEPCCWMTYRQHRDAEEALDIFESPDGGGSGAGPSDEAGDDERELALQRLGPHEGGAGHGAGSGGCRGWQPRMWALFEDPYSSRAARVVAFASLFFILVSITTFCLETHEAFNIDRNVTEILRVGNITSVHFRREVETEPILTYIEGVCVLWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKRKKHVPRPAQLESPMYCKSEETSPRDSTCSDTSPPAREEGMIERKRADSKQNGDANAVLSDEEGAGLTQPLASSPTPEERRALRRSTTRDRNKKAAACFLLSTGDYACADGSVRKGTFVLRDLPLQHSPEAACPPTAGTLFLPH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Chicken

100

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03721 As Substrate

Q03721

Site	PTM Type	Enzyme	Source
S8	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S9	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S15	Phosphorylation	P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA)	Uniprot
S21	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB) , P17252 (PRKCA)	Uniprot
T49	Phosphorylation		Uniprot
Y50	Phosphorylation		Uniprot
Y597	Phosphorylation		Uniprot

Research Backgrounds

Q03721_KCNC4_HUMAN

Function:

This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

PTMs:

Phosphorylation of serine residues in the inactivation gate inhibits rapid channel closure.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Homotetramer (Probable). Heterotetramer of potassium channel proteins (By similarity).

Family&Domains:

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily.

References

1). β-Catenin Controls the Electrophysiologic Properties of Skeletal Muscle Cells by Regulating the α2 Isoform of Na+/K+-ATPase. Frontiers in Neuroscience, 2019 (PubMed: 31440132) [IF=4.3]

Application: WB Species: mouse Sample: C2C12 cells

FIGURE 3 | Protein expression of α2NKA, α1NKA, and Kv3.4 in each transfection group. (A) Representative expressions of α1NKA, α2NKA, β-catenin, Kv3.4 and GAPDH proteins in five independent groups respectively

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Kv3.4/KCNC4 Antibody - #DF10312