Twist1 Antibody | Affinity Biosciences

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Figure 3:| Assessment of the protein levels of IGF1 and TWIST1 in MCF-7 cells using Western blot analysis.

Product:	Twist1 Antibody
Catalog:	AF7945
Description:	Rabbit polyclonal antibody to Twist1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Sheep, Chicken
Mol.Wt.:	21kDa; 21kD(Calculated).
Uniprot:	Q15672
RRID:	AB_2844308

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

Twist1 Antibody detects endogenous levels of total Twist1.

RRID:

AB_2844308
Cite Format: Affinity Biosciences Cat# AF7945, RRID:AB_2844308.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; TWIST; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; TWIST1; TWST1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q15672(TWST1_HUMAN) >>Visit HPA database.

Gene(ID):

TWIST1(7291)

Expression:

Q15672 TWST1_HUMAN:

Subset of mesodermal cells.

Sequence:

Q15672 TWST1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Chicken

Horse

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15672 As Substrate

Q15672

Site	PTM Type	Enzyme	Source
S6	Phosphorylation	P28482 (MAPK1)	Uniprot
S18	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S20	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K38	Ubiquitination		Uniprot
S42	Phosphorylation	P31749 (AKT1)	Uniprot
S45	Phosphorylation		Uniprot
S68	Phosphorylation	Q15759 (MAPK11) , P45983 (MAPK8) , Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
K73	Acetylation		Uniprot
S102	Phosphorylation		Uniprot
Y103	Phosphorylation		Uniprot
T121	Phosphorylation		Uniprot
S123	Phosphorylation	P31749 (AKT1)	Uniprot
T137	Phosphorylation		Uniprot
K142	Ubiquitination		Uniprot
S144	Phosphorylation		Uniprot
K145	Ubiquitination		Uniprot

Research Backgrounds

Q15672_TWST1_HUMAN

Function:

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Subset of mesodermal cells.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). RCC2 Expression Stimulates ER-Positive Breast Tumorigenesis. Journal of Oncology, 2020 (PubMed: 32565805)

Application: WB Species: Sample: MCF-7 cells

Figure 3:| Assessment of the protein levels of IGF1 and TWIST1 in MCF-7 cells using Western blot analysis. MCF-7 cells were transfected with anti-RCC2 siRNA (si-RCC2) (a–c) or RCC2-expressing plasmids (O-RCC2) (d–f ).

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Twist1 Antibody - #AF7945