SSH3 Antibody | Affinity Biosciences

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Western blot analysis of SSH3 in lysates of HuvEc, using SSH3 Antibody(AF7924).

Product:	SSH3 Antibody
Catalog:	AF7924
Description:	Rabbit polyclonal antibody to SSH3
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	74KD; 73kD(Calculated).
Uniprot:	Q8TE77
RRID:	AB_2844287

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(91%), Sheep(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

SSH3 Antibody detects endogenous levels of total SSH3.

RRID:

AB_2844287
Cite Format: Affinity Biosciences Cat# AF7924, RRID:AB_2844287.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10928; FLJ20515; hSSH 3L; hSSH-3L; hSSH3L; Protein phosphatase Slingshot homolog 3; Slingshot 3; Slingshot homolog 3; Slingshot3; SSH 3; SSH 3L; SSH-3L; SSH-like protein 3; Ssh3; SSH3_HUMAN; SSH3L;

Immunogens

Immunogen:

Uniprot:

Q8TE77(SSH3_HUMAN) >>Visit HPA database.

Gene(ID):

SSH3(54961)

Sequence:

Q8TE77 SSH3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEASSEPTEKAPSEEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAPRPPRLRYLLVVSTREGEGLSQDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGGFSVTSGGQSRIFKPISIQTMWATLQVLHQACEAALGSGLVPGGSALTWASHYQERLNSEQSCLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELWKVLDVSDLESVTSKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRSAATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTASRQSHVWEQKVGGVSPEEHPAPEVSTPFPPLPPEPEGGGEEKVVGMEESQAAPKEEPGPRPRINLRGVMRSISLLEPSLELESTSETSDMPEVFSSHESSHEEPLQPFPQLARTKGGQQVDRGPQPALKSRQSVVTLQGSAVVANRTQAFQEQEQGQGQGQGEPCISSTPRFRKVVRQASVHDSGEEGEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TE77 As Substrate

Q8TE77

Site	PTM Type	Enzyme	Source
T5	Phosphorylation		Uniprot
S9	Phosphorylation		Uniprot
S13	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
T17	Phosphorylation		Uniprot
S37	Phosphorylation	Q96GD4 (AURKB)	Uniprot
K67	Acetylation		Uniprot
S70	Phosphorylation		Uniprot
T79	Phosphorylation		Uniprot
S85	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
K90	Ubiquitination		Uniprot
S259	Phosphorylation		Uniprot
K281	Ubiquitination		Uniprot
S290	Phosphorylation		Uniprot
T292	Phosphorylation		Uniprot
S293	Phosphorylation		Uniprot
K294	Ubiquitination		Uniprot
S385	Phosphorylation		Uniprot
S484	Phosphorylation		Uniprot
K523	Sumoylation		Uniprot
S547	Phosphorylation		Uniprot
K598	Methylation		Uniprot
S602	Phosphorylation		Uniprot
T605	Phosphorylation		Uniprot
S609	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
T638	Phosphorylation		Uniprot
S649	Phosphorylation		Uniprot
S653	Phosphorylation		Uniprot

Research Backgrounds

Q8TE77_SSH3_HUMAN

Function:

Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.

Subunit Structure:

Does not bind to, or colocalize with, filamentous actin.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

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SSH3 Antibody - #AF7924