Product: NT5C Antibody
Catalog: AF7922
Description: Rabbit polyclonal antibody to NT5C
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: Q8TCD5
RRID: AB_2844285

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NT5C Antibody detects endogenous levels of total NT5C.
RRID:
AB_2844285
Cite Format: Affinity Biosciences Cat# AF7922, RRID:AB_2844285.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3''-pyrimidine nucleotidase; 5' nucleotidase, deoxy (pyrimidine); 5' nucleotidase, deoxy (pyrimidine), cytosolic type C; 5''(3'')-deoxyribonucleotidase; 5'(3')-deoxyribonucleotidase, cytosolic type; 5', 3'-nucleotidase, cytosolic; 5-prime,3-prime-nucleotidase, cytosolic; cdN; Cytosolic 5''; cytosolic 5',3'-pyrimidine nucleotidase; cytosolic type; cytosolic type C,5',3'-nucleotidase; cytosolic,uridine 5'-monophosphate phosphohydrolase 2; Deoxy 5' nucleotidase 1; Deoxy-5''-nucleotidase 1; Deoxyribonucleotidase, cytosolic, 1; DNT; dNT-1; dNT1; Nt5c; NT5C_HUMAN; P5N2; PN-I; PN-II; Pyrimidine 5-prime nucleotidase 2; UMPH2; uridine 5'-monophosphate phosphohydrolase 2; uridine 5-prime monophosphate hydrolase 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8TCD5 NT5C_HUMAN:

Detected in skeletal muscle, heart and pancreas.

Sequence:
MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVLGDLLIDDKDTVRGQEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TCD5 As Substrate

Site PTM Type Enzyme
S63 Phosphorylation
Y65 Phosphorylation
K112 Acetylation
K112 Ubiquitination
K134 Ubiquitination
K146 Acetylation
K146 Ubiquitination
S182 Phosphorylation
S184 Phosphorylation
K194 Ubiquitination

Research Backgrounds

Function:

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in skeletal muscle, heart and pancreas.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the 5'(3')-deoxyribonucleotidase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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